基孔肯雅病毒的感染性病毒定量——病毒蚀斑测定法
Infectious Viral Quantification of Chikungunya Virus-Virus Plaque Assay.
作者信息
Kaur Parveen, Lee Regina Ching Hua, Chu Justin Jang Hann
机构信息
Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore.
出版信息
Methods Mol Biol. 2016;1426:93-103. doi: 10.1007/978-1-4939-3618-2_9.
The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via virus plaque assays.
蚀斑测定法是定量检测传染性病毒滴度的重要方法。用病毒颗粒感染的细胞被覆盖上一层粘性底物。经过适当的孵育期会形成蚀斑，可对其进行固定和染色以便观察。在此，我们描述一种通过病毒蚀斑测定法来测量基孔肯雅病毒（CHIKV）滴度的方法。
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