基孔肯雅病毒感染的中和试验：蚀斑减少中和试验
Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test.
作者信息
Azami Nor Azila Muhammad, Moi Meng Ling, Takasaki Tomohiko
机构信息
Department of Virology 1, National Institute of Infectious Diseases, Toyama 1-23-1 Shinjuku-ku, Tokyo, 162-8640, Japan.
Graduate School for Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
出版信息
Methods Mol Biol. 2016;1426:273-82. doi: 10.1007/978-1-4939-3618-2_25.
Neutralization assay is a technique that detects and quantifies neutralizing antibody in serum samples by calculating the percentage of reduction of virus activity, as the concentration of virus used is usually constant. Neutralizing antibody titer is conventionally determined by calculating the percentage reduction in total virus infectivity by counting and comparing number of plaques (localized area of infection due to cytopathic effect) with a standard amount of virus. Conventional neutralizing test uses plaque-reduction neutralization test (PRNT) to determine neutralizing antibody titers against Chikungunya virus (CHIKV). Here we describe the plaque reduction neutralization assay (PRNT) using Vero cell lines to obtain neutralizing antibody titers.
中和试验是一种通过计算病毒活性降低的百分比来检测和定量血清样本中中和抗体的技术，因为所用病毒的浓度通常是恒定的。中和抗体滴度通常是通过计算总病毒感染性的降低百分比来确定的，即通过计数并将蚀斑（由于细胞病变效应导致的局部感染区域）数量与标准量的病毒进行比较。传统的中和试验使用蚀斑减少中和试验（PRNT）来确定针对基孔肯雅病毒（CHIKV）的中和抗体滴度。在此，我们描述了使用Vero细胞系进行蚀斑减少中和试验（PRNT）以获得中和抗体滴度的方法。
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