The Jenner Institute, Nuffield Department of Medicine, University of Oxford, The Henry Wellcome Building for Molecular Physiology, Roosevelt Drive, Oxford OX3 7DQ, UK.
Division of Structural Biology, University of Oxford, Wellcome Centre for Human Genetics, Roosevelt Drive, Oxford, UK.
Viruses. 2019 May 1;11(5):407. doi: 10.3390/v11050407.
Chikungunya fever is a debilitating disease caused by Chikungunya virus (CHIKV) that can result in long-lasting arthralgias. The early diagnosis of CHIKV relies on PCR during the acute infection phase to allow differential diagnosis with other co-circulating arboviruses such as dengue and Zika. Alternatively, serology can support diagnosis and provide epidemiological information on current and past outbreaks. Many commercial serological ELISA assays are based on the inactivated whole CHIKV, but their sensitivity and specificity show great variability. We produced recombinant CHIKV E2 that is suitable for ELISA assays, which was used for the serodiagnosis of CHIKV infections occurring in an arbovirus endemic Mexican region within Michoacán state. A cross-sectional study was conducted in 2016-2017; sera was obtained from 15 healthy donors and 68 patients presenting undifferentiated febrile illness. Serum samples were screened by RT-PCR and by our in-house ELISA assay. Our results indicate that IgM and IgG anti-CHIKV E2 antibodies were detected with our ELISA assay with higher sensitivity than a commercially available CHIKV ELISA kit. Our simple and sensitive ELISA assay for the serodiagnosis of CHIKV infections can be applied to population-based seroprevalence surveys and has potential for monitoring vaccine immunogenicity in CHIKV vaccine clinical trials.
基孔肯雅热是一种由基孔肯雅病毒（CHIKV）引起的使人虚弱的疾病，可导致长期关节痛。CHIKV 的早期诊断依赖于急性感染期的 PCR，以允许与其他同时流行的虫媒病毒（如登革热和寨卡病毒）进行鉴别诊断。或者，血清学可以支持诊断，并提供有关当前和过去暴发的流行病学信息。许多商业血清学 ELISA 检测试剂盒基于灭活的整个 CHIKV，但它们的敏感性和特异性显示出很大的可变性。我们生产了适合 ELISA 检测的重组 CHIKV E2，用于在墨西哥米却肯州的虫媒病毒流行地区发生的 CHIKV 感染的血清学诊断。2016-2017 年进行了一项横断面研究；从 15 名健康供体和 68 名出现未分化发热病的患者中获得血清。血清样本通过 RT-PCR 和我们的内部 ELISA 检测进行筛查。我们的结果表明，与市售的 CHIKV ELISA 试剂盒相比，我们的 ELISA 检测法检测到 IgM 和 IgG 抗-CHIKV E2 抗体的敏感性更高。我们用于 CHIKV 感染血清学诊断的简单而敏感的 ELISA 检测法可应用于基于人群的血清流行率调查，并有可能监测 CHIKV 疫苗临床试验中的疫苗免疫原性。
