一种用于智能手机监测人呼出气冷凝物中HO的化学发光平台。
A chemiluminescent platform for smartphone monitoring of HO in human exhaled breath condensates.
作者信息
Quimbar Miguel E, Krenek Katherine M, Lippert Alexander R
机构信息
Department of Chemistry, Southern Methodist University, Dallas, TX 75275-0314, USA.
Department of Chemistry, Southern Methodist University, Dallas, TX 75275-0314, USA; Center for Drug Discovery, Design, and Delivery (CD4), Southern Methodist University, Dallas, TX 75275-0314, USA; Center for Global Health Impact (CGHI), Southern Methodist University, Dallas, TX 75275-0314, USA.
出版信息
Methods. 2016 Oct 15;109:123-130. doi: 10.1016/j.ymeth.2016.05.017. Epub 2016 May 24.
Noninvasive measurement of oxidative markers in clinical samples has the potential to rapidly provide information for disease management, but is limited by the need for expensive analytical instrumentation that precludes home monitoring or point-of-care applications. We have developed a simple to use diagnostic platform for airway hydrogen peroxide (HO) that combines optimized reaction-based chemiluminescent designs with an inexpensive home-built darkbox and readily available smartphone cameras. Specialized photography software applications and analysis of pixel intensity enables quantification of sample concentrations. Using this platform, sample HO concentrations as low as 264nM can be detected. The platform has been used to measure HO in the exhaled breath condensates of human subjects, showing good agreement with the standard Amplex Red assay.
对临床样本中的氧化标志物进行无创测量，有潜力为疾病管理快速提供信息，但受到昂贵分析仪器的限制，这排除了家庭监测或即时检测应用。我们开发了一种用于气道过氧化氢（HO）的简单易用诊断平台，该平台将基于反应的优化化学发光设计与廉价的自制暗箱和现成的智能手机摄像头相结合。专门的摄影软件应用和像素强度分析能够对样本浓度进行定量。使用该平台，可以检测低至264nM的样本HO浓度。该平台已用于测量人类受试者呼出气冷凝物中的HO，与标准的Amplex Red检测方法显示出良好的一致性。
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