Desjardins M, Bendayan M
Département d'Anatomie, Université de Montréal, Québec, Canada.
J Histochem Cytochem. 1989 Jun;37(6):885-97. doi: 10.1177/37.6.2723404.
Type IV collagen, entactin, heparan sulfate proteoglycan, and laminin antigenic sites were revealed on various rat renal basement membranes by use of protein A-gold immunocytochemistry. The basement membranes of the proximal and distal convoluted tubules, those of Bowman's capsule and glomerulus, and the mesangial matrix were labeled for all the antigens but to differing extents. Control experiments confirmed the specificity of these labelings. Quantitative evaluation revealed an important heterogeneity for each antigen among the various basement membranes. This heterogeneity suggests that the basement membrane components must arrange themselves in different ways, possibly to account for differences in functional properties of the various renal structures.
通过蛋白A-金免疫细胞化学方法，在各种大鼠肾基底膜上发现了IV型胶原、巢蛋白、硫酸乙酰肝素蛋白聚糖和层粘连蛋白抗原位点。近端和远端曲管的基底膜、鲍曼囊和肾小球的基底膜以及系膜基质均被标记上所有抗原，但程度不同。对照实验证实了这些标记的特异性。定量评估显示，各种基底膜中每种抗原都存在重要的异质性。这种异质性表明，基底膜成分必定以不同方式排列，可能是为了解释各种肾结构功能特性的差异。
