Nishimura K, Takada K, Noguchi Y, Yamaoka A
J Electron Microsc (Tokyo). 1989;38(1):16-23.
We examined whether or not gingival fibroblasts attach to curetted cementum surface in periodontally involved teeth. For in vitro experiment, superficial cementum in periodontally involved human teeth was curetted and cut into 3 X 3 mm fragments, put into a plastic plate and mixed with fibroblasts for 1 to 3 days. Numerous fibroblasts attached to the curetted cementum surfaces, while only a few fibroblasts could attach to the diseased cementum. For in vivo experiment, experimental periodontitis was induced in monkeys by leaving a resin plate in artificially created marginal bone crest for 3 months. In the flap surgery, only superficial cementum was curetted. Two- and 4-month biopsy specimens disclosed that the regenerated gingival tissues attached well to the curetted cementum surface. Both in vivo and in vitro results indicated subjacent cementum in periodontally involved teeth could be a suitable surface for fibrous attachment.
我们研究了牙龈成纤维细胞是否会附着在牙周病患牙的刮治牙骨质表面。在体外实验中，刮除牙周病患牙的表层牙骨质并切成3×3毫米的碎片，放入塑料培养皿中，与成纤维细胞混合培养1至3天。大量成纤维细胞附着在刮治后的牙骨质表面，而只有少数成纤维细胞能附着在病变牙骨质上。在体内实验中，通过在人工制造的边缘牙槽嵴处放置树脂板3个月，诱导猴子患上实验性牙周炎。在翻瓣手术中，仅刮除表层牙骨质。术后2个月和4个月的活检标本显示，再生的牙龈组织与刮治后的牙骨质表面附着良好。体内和体外实验结果均表明，牙周病患牙的下方牙骨质可能是纤维附着的合适表面。
