Babay N
Division of Periodontics, Department of Preventive Dental Sciences, King Saud University College of Dentistry, P.O. Box 60169, Riyadh 11545, Kingdom of Saudi Arabia.
Braz Dent J. 2001;12(1):17-21.
This study evaluated, in vitro, fibroblast attachment to periodontally involved root surfaces which were either root planed or acid/chelated by different agents. Specimens were divided into 3 groups of 12 specimens each. The root surfaces were root planed with a Gracey 7/8 curette, an EMS or an Amdent piezo-electric scaler and treated with saline, citric acid, tetracycline hydrochloride or EDTA to produce different surface textures. They were then cultured with fibroblasts for 72 h and examined by scanning electron microscopy. There was a significantly greater number of fibroblasts attached to specimens treated with citric acid, tetracycline and EDTA than to those root planed only. Furthermore, fibroblasts were more likely to attach to rough-surfaced than to smooth-surfaced specimens.
本研究在体外评估了成纤维细胞与牙周受累根面的附着情况，这些根面分别进行了根面平整或用不同试剂进行酸处理/螯合处理。标本分为3组，每组12个标本。用Gracey 7/8刮治器、EMS或Amdent压电洁治器对根面进行根面平整，并用生理盐水、柠檬酸、盐酸四环素或乙二胺四乙酸（EDTA）处理，以产生不同的表面纹理。然后将它们与成纤维细胞一起培养72小时，并通过扫描电子显微镜进行检查。与仅进行根面平整的标本相比，用柠檬酸、四环素和EDTA处理的标本附着的成纤维细胞数量明显更多。此外，成纤维细胞更倾向于附着在粗糙表面的标本上，而不是光滑表面的标本上。
