Fujitani Y, Higaki S, Sawada H, Hirosawa K
J Electron Microsc (Tokyo). 1989;38(1):34-40.
We have visualized the nuclear pore complex (NPCx) three-dimensionally by quick-freeze, deep etching and suggest that NPCx is composed of four rings and associated filaments. The rings were located at the cytoplasmic side, at the nucleoplasmic side, and at the pore waist as well. Some of the filaments emanated toward the cytoplasm, some sprawled over the cytoplasmic face of the nuclear membrane, and some intervened the rings. The emanating filaments were considered to be vimentin filaments judging from the replica and thin-sectioned image.
我们通过快速冷冻、深度蚀刻对核孔复合体（NPCx）进行了三维可视化，并认为NPCx由四个环和相关的细丝组成。这些环分别位于细胞质侧、核质侧以及孔腰处。一些细丝伸向细胞质，一些在核膜的细胞质面伸展，还有一些介于环之间。从复制品和超薄切片图像判断，伸向细胞质的细丝被认为是波形蛋白细丝。
