Fiorini D, Boarelli M C
School of Science and Technology, Chemistry Division, University of Camerino, V. S. Agostino 1, I-62032 Camerino, MC, Italy.
School of Science and Technology, Chemistry Division, University of Camerino, V. S. Agostino 1, I-62032 Camerino, MC, Italy.
J Chromatogr A. 2016 Jul 1;1453:134-7. doi: 10.1016/j.chroma.2016.05.045. Epub 2016 May 12.
When hydrogen is used as carrier gas, carbon-carbon double bonds may be hydrogenated in the hot gas chromatograph (GC) injector if introduced by solid-phase microextraction (SPME). SPME fibers coated with polydimethylsiloxane (PDMS)/carboxen/divinylbenzene (DVB), PDMS/carboxen, polyacrylate, PDMS/DVB and PDMS on fused silica, stableflex or metal alloy core have been tested with fatty acid methyl esters (FAMEs) from olive oil. Using coatings containing DVB, hydrogenation took place with high conversion rates (82.0-92.9%) independently of the core material. With all fibers having a metal core, hydrogenation was observed to a certain extent (27.4-85.3%). PDMS, PDMS/carboxen and polyacrylate coated fibers with a fused silica or stableflex core resulted in negligible hydrogenation (0.2-2.5%). The occurrence of hydrogenation was confirmed also with other substances containing carbon-carbon double bonds (n-alkenes, alkenoic acids, mono- and polyunsaturated fatty acid methyl and ethyl esters).
当使用氢气作为载气时，如果通过固相微萃取（SPME）引入，碳 - 碳双键可能会在热气相色谱仪（GC）进样器中被氢化。已使用涂覆有聚二甲基硅氧烷（PDMS）/碳分子筛/二乙烯基苯（DVB）、PDMS/碳分子筛、聚丙烯酸酯、PDMS/DVB以及在熔融石英、稳定柔性或金属合金芯上的PDMS的SPME纤维对橄榄油中的脂肪酸甲酯（FAMEs）进行了测试。使用含有DVB的涂层时，无论芯材如何，氢化反应都以高转化率（82.0 - 92.9%）发生。对于所有具有金属芯的纤维，都观察到了一定程度的氢化（27.4 - 85.3%）。涂覆有PDMS、PDMS/碳分子筛和聚丙烯酸酯且芯为熔融石英或稳定柔性的纤维导致的氢化可忽略不计（0.2 - 2.5%）。含有碳 - 碳双键的其他物质（正烯烃、烯酸、单不饱和和多不饱和脂肪酸甲酯和乙酯）也证实了氢化现象的发生。
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