通过噬菌体展示技术生成抗毒素和病毒的重组抗体用于诊断和治疗
Generation of Recombinant Antibodies Against Toxins and Viruses by Phage Display for Diagnostics and Therapy.
作者信息
Unkauf Tobias, Miethe Sebastian, Fühner Viola, Schirrmann Thomas, Frenzel André, Hust Michael
机构信息
Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
YUMAB GmbH, Rebenring 33, 38106, Braunschweig, Germany.
出版信息
Adv Exp Med Biol. 2016;917:55-76. doi: 10.1007/978-3-319-32805-8_4.
Abstract
Antibody phage display is an in vitro technology to generate recombinant antibodies. In particular for pathogens like viruses or toxins, antibody phage display is an alternative to hybridoma technology, since it circumvents the limitations of the immune system. Phage display allows the generation of human antibodies from naive antibody gene libraries when either immunized patients are not available or immunization is not ethically feasible. This technology also allows the construction of immune libraries to select in vivo affinity matured antibodies if immunized patients or animals are available.In this review, we describe the generation of human and human-like antibodies from naive antibody gene libraries and antibodies from immune antibody gene libraries. Furthermore, we give an overview about phage display derived recombinant antibodies against viruses and toxins for diagnostics and therapy.
摘要
抗体噬菌体展示是一种用于产生重组抗体的体外技术。特别是对于病毒或毒素等病原体，抗体噬菌体展示是杂交瘤技术的一种替代方法，因为它规避了免疫系统的局限性。当无法获得免疫患者或免疫在伦理上不可行时，噬菌体展示允许从天然抗体基因文库中产生人源抗体。如果有免疫患者或动物，该技术还允许构建免疫文库以选择体内亲和力成熟的抗体。在本综述中，我们描述了从天然抗体基因文库中产生人源抗体和类人源抗体以及从免疫抗体基因文库中产生抗体的方法。此外，我们概述了用于诊断和治疗的噬菌体展示衍生的抗病毒和抗毒素重组抗体。
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