London J A, Cohen L B, Wu J Y
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510.
J Neurosci. 1989 Jun;9(6):2182-90. doi: 10.1523/JNEUROSCI.09-06-02182.1989.
Optical recording methods using voltage-sensitive dyes were used to monitor activity in rat somatosensory cortex. We measured evoked signals in response to whisker stimulation before (control) and after the addition of the epileptogenic agent, bicuculline, and also detected spontaneous interictal events that occurred after bicuculline. Bicuculline led to an increase in the size, duration, cortical extent, and, surprisingly, the latency of the evoked responses. These enhanced evoked responses appeared to originate in the region of the control response and propagate outward. In contrast, the spontaneous signals appeared to originate at random cortical positions and had a more variable cortical extent. A transition signal measured just after the addition of bicuculline was larger than the control response but localized and rapid in time course. In most cases, there was a good correlation between the optical recordings and field potential measurements made with a ball electrode on the cortical surface, but there were occasional instances where the optical signal disappeared while the ball electrode signal was unchanged.
使用电压敏感染料的光学记录方法被用于监测大鼠体感皮层的活动。我们在添加致痫剂荷包牡丹碱之前（对照）和之后测量了对触须刺激的诱发信号，并且还检测了荷包牡丹碱添加后发生的自发性发作间期事件。荷包牡丹碱导致诱发反应的大小、持续时间、皮层范围增加，令人惊讶的是，还导致诱发反应的潜伏期增加。这些增强的诱发反应似乎起源于对照反应区域并向外传播。相比之下，自发性信号似乎起源于随机的皮层位置，并且皮层范围更具变异性。在添加荷包牡丹碱后立即测量的过渡信号比对照反应大，但在时间进程上是局部的且快速的。在大多数情况下，光学记录与在皮层表面使用球形电极进行的场电位测量之间有很好的相关性，但偶尔也会出现光学信号消失而球形电极信号不变的情况。
