Orbach H S, Cohen L B, Grinvald A
J Neurosci. 1985 Jul;5(7):1886-95. doi: 10.1523/JNEUROSCI.05-07-01886.1985.
We have investigated the use of optical methods for monitoring neuron activity in mammalian cortex. The cortex was stained with a voltage-sensitive dye and fluorescence was simultaneously measured from 124 areas using a photodiode array. Optical signals were detected in rat somatosensory cortex in response to small whisker movements and in visual cortex in response to light flashes to the eye. Relatively large signals were obtained during focal interictal epileptiform discharges induced by bicuculline. The measuring system had a time resolution of milliseconds and a spatial resolution of a few hundred micrometers. Simultaneous, multi-site optical recordings of activity may provide a new and potentially powerful method for studying function and dysfunction in mammalian cortex.
我们研究了利用光学方法监测哺乳动物皮层中神经元的活动。用一种电压敏感染料对皮层进行染色，并使用光电二极管阵列同时从124个区域测量荧光。在大鼠体感皮层中，对轻微的触须运动有光学信号检测；在视觉皮层中，对眼睛的闪光有光学信号检测。在荷包牡丹碱诱发的局灶性发作间期癫痫样放电期间获得了相对较大的信号。该测量系统的时间分辨率为毫秒级，空间分辨率为几百微米。同时进行的多部位光学活动记录可能为研究哺乳动物皮层的功能和功能障碍提供一种新的、潜在强大的方法。
