Cheng S Q, Zhou X D, Tang Z Y, Yu Y, Bao S S, Qian D C
Shu-Qun Cheng, Xin-Da Zhou, Zhao-You Tang, Yao Yu, Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China.
World J Gastroenterol. 1997 Sep 15;3(3):134-6. doi: 10.3748/wjg.v3.i3.134.
To observe the ultrastructural changes of liver tissues on normal rabbit ablated by high-intensity focused ultrasound (HIFU).
A single shot of 1.1 MHz focused ultrasound at an intensity of 500 W/cm(2) with 20-s duration of continuous exposure was applied intraoperatively in normal rabbit livers. Ultrastructural changes of the sonoablated lesion, as viewed by light and electron microscopy, were observed.
Liver cells at the center of the sonoablated lesion showed irreversible degeneration immediately after HIFU treatment; electron microscopy showed that although the liver cells appeared normal histologically, irregularly shaped cavities of about 0.3-0.5 μm in diameter were present in the cytoplasm.
Thermal damages may be the main mechanism of HIFU-induced ablation of liver tissues besides cavitation effect.
观察高强度聚焦超声（HIFU）消融正常兔肝脏后肝组织的超微结构变化。
术中对正常兔肝脏单次施加1.1MHz聚焦超声，强度为500W/cm²，持续暴露20秒。通过光镜和电镜观察超声消融病灶的超微结构变化。
HIFU治疗后，超声消融病灶中心的肝细胞立即出现不可逆性变性；电镜显示，尽管肝细胞在组织学上看似正常，但细胞质中存在直径约0.3 - 0.5μm的不规则形空泡。
除空化效应外，热损伤可能是HIFU诱导肝组织消融的主要机制。
