Chen Dongsheng, Zeng Nan, Wang Yunfei, He Honghui, Tuchin Valery V, Ma Hui
Tsinghua University, Graduate School at Shenzhen, Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, 2279 Lishui Road, Shenzhen 518055, ChinabTsinghua University, Department of Physics, 30 Shuangqi.
Tsinghua University, Graduate School at Shenzhen, Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, 2279 Lishui Road, Shenzhen 518055, China.
J Biomed Opt. 2016 Aug;21(8):081209. doi: 10.1117/1.JBO.21.8.081209.
We conducted Monte Carlo simulations based on anisotropic sclera-mimicking models to examine the polarization features in Mueller matrix polar decomposition (MMPD) parameters during the refractive index matching process, which is one of the major mechanisms of optical clearing. In a preliminary attempt, by changing the parameters of the models, wavelengths, and detection geometries, we demonstrate how the depolarization coefficient and retardance vary during the refractive index matching process and explain the polarization features using the average value and standard deviation of scattering numbers of the detected photons. We also study the depth-resolved polarization features during the gradual progression of the refractive index matching process. The results above indicate that the refractive index matching process increases the depth of polarization measurements and may lead to higher contrast between tissues of different anisotropies in deeper layers. MMPD-derived polarization parameters can characterize the refractive index matching process qualitatively.
我们基于各向异性巩膜模拟模型进行了蒙特卡罗模拟，以研究在光学清除的主要机制之一——折射率匹配过程中，穆勒矩阵偏振分解（MMPD）参数中的偏振特征。在初步尝试中，通过改变模型参数、波长和检测几何结构，我们展示了折射率匹配过程中去偏振系数和延迟是如何变化的，并利用检测到的光子散射数的平均值和标准差来解释偏振特征。我们还研究了折射率匹配过程逐渐推进时的深度分辨偏振特征。上述结果表明，折射率匹配过程增加了偏振测量的深度，并可能导致更深层中不同各向异性组织之间具有更高的对比度。MMPD衍生的偏振参数可以定性地表征折射率匹配过程。
