Zhu Lina, She Tiantian, Zhang Yingying, Li Shaoshen, Xu Zixian, Yan Juanjuan, Li Huiqiang
School of Medical Laboratory, Tianjin Medical University, Tianjin, China.
Department of Medical Laboratory, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, China.
Mol Nutr Food Res. 2016 Oct;60(10):2275-2287. doi: 10.1002/mnfr.201600144. Epub 2016 Jun 23.
Crab is a major source of shellfish allergens. Most studies have focused on allergens in crab muscle (CM) rather than on allergens in crab ovary (CO). This study aimed to identify potential allergens in CO from Eriocheir sinensis.
Dot blot and immunoblotting results revealed the differential reactivity of CM and CO extracts to positive sera from patients allergic to crabs. Four CO proteins showed good specific IgE-binding activities in 2-DE/immunoblotting analysis; mass spectrometry identified the proteins as hemocyanin, vitellogenin, ovary development-related protein EJO1and EJO2. The recently identified allergen EJO1 is named 'Eri s 2' in the World Health Organization and International Union of Immunological Societies (WHO/IUIS) allergen nomenclature database. Recombinant Eri s 2 exhibited good reactivity to positive sera, and pre-incubation with recombinant Eri s 2 abrogated the reactivity of positive sera from two patients to CO in a dose-dependent manner. Moreover, co-incubation of recombinant Eri s 2 with patient basophils dose-dependently promoted basophil activation, confirming the biological activity of Eri s 2.
CO tissue is an important allergen source, and Eri s 2 is a new crab allergen. This study provides insights that will be useful for component-resolved diagnostics for crab allergy.
蟹是贝类过敏原的主要来源。大多数研究集中在蟹肉（CM）中的过敏原，而非蟹卵巢（CO）中的过敏原。本研究旨在鉴定中华绒螯蟹卵巢中的潜在过敏原。
斑点印迹和免疫印迹结果显示，CM和CO提取物与对蟹过敏患者的阳性血清的反应性存在差异。在二维电泳/免疫印迹分析中，四种CO蛋白显示出良好的特异性IgE结合活性；质谱鉴定这些蛋白为血蓝蛋白、卵黄蛋白原、卵巢发育相关蛋白EJO1和EJO2。最近鉴定出的过敏原EJO1在世界卫生组织和国际免疫学会（WHO/IUIS）过敏原命名数据库中被命名为“Eri s 2”。重组Eri s 2对阳性血清表现出良好的反应性，并且与重组Eri s 2预孵育以剂量依赖的方式消除了两名患者阳性血清对CO的反应性。此外，重组Eri s 2与患者嗜碱性粒细胞共同孵育以剂量依赖的方式促进嗜碱性粒细胞活化，证实了Eri s 2的生物学活性。
CO组织是重要的过敏原来源，Eri s 2是一种新的蟹过敏原。本研究提供的见解将有助于蟹过敏的组分解析诊断。
