Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 11529 Taiwan.
Sci Rep. 2016 May 31;6:26785. doi: 10.1038/srep26785.
Electrospray ionization-mass spectrometry (ESI-MS) is used to analyze metal species in a variety of samples. Here, we describe an application for identifying metal species by tandem mass spectrometry (ESI-MS/MS) with the release of free metals from the corresponding metal-ligand complexes. The MS/MS data were used to elucidate the possible fragmentation pathways of different metal-deoxymugineic acid (-DMA) and metal-nicotianamine (-NA) complexes and select the product ions with highest abundance that may be useful for quantitative multiple reaction monitoring. This method can be used for identifying different metal-ligand complexes, especially for metal species whose mass spectra peaks are clustered close together. Different metal-DMA/NA complexes were simultaneously identified under different physiological pH conditions with this method. We further demonstrated the application of the technique for different plant samples and with different MS instruments.
电喷雾电离-质谱（ESI-MS）用于分析各种样品中的金属物种。在这里，我们描述了一种应用，通过从相应的金属配体络合物中释放游离金属来通过串联质谱（ESI-MS/MS）来鉴定金属物种。MS/MS 数据用于阐明不同金属-脱氧麦根碱（-DMA）和金属-烟碱胺（-NA）络合物的可能碎裂途径，并选择丰度最高的产物离子，这些离子可能对定量多重反应监测有用。该方法可用于鉴定不同的金属配体络合物，特别是对于其质谱峰聚集在一起的金属物种。使用该方法可以在不同的生理 pH 条件下同时鉴定不同的金属-DMA/NA 络合物。我们进一步展示了该技术在不同植物样品和不同 MS 仪器上的应用。
