采用微浅表增强脂肪注射液（M-SEFFI）进行眼周和口周皮肤老化的皮肤年轻化及容积增加治疗

Skin Rejuvenation and Volume Enhancement with the Micro Superficial Enhanced Fluid Fat Injection (M-SEFFI) for Skin Aging of the Periocular and Perioral Regions.

作者信息

Gennai Alessandro, Zambelli Alessandra, Repaci Erica, Quarto Rodolfo, Baldelli Ilaria, Fraternali Giulio, Bernardini Francesco P

机构信息

Dr Gennai is a plastic surgeon in private practice in Bologna and Milan, Italy. Drs Zambelli and Bernardini are oculoplastic surgeons in private practice in Genova, Italy. Dr Repaci is a Researcher and Dr Quarto is the Chairman, Department of Experimental Medicine, University of Genova, Genova, Italy. Dr Baldelli is a Plastic Surgeon, Department of Integrated Surgical and Diagnostic Sciences, University of Genova, Genova, Italy. Dr Fraternali is a Pathologist, Department of Pathology, University of Genova, Genova, Italy

出版信息

Aesthet Surg J. 2017 Jan;37(1):14-23. doi: 10.1093/asj/sjw084. Epub 2016 May 30.

DOI:10.1093/asj/sjw084

PMID:27241362

Abstract

BACKGROUND

Adipose-derived stromal and stem cells (ADSC) in autologous fat promises regenerative advantages, and injected into the dermal and subdermal layers, enhances rejuvenation and volume. However, extremely superficial fat injection with current techniques is limited.

OBJECTIVES

Efficacy and viability evaluation of fat harvested with extremely small side port (0.3 mm) cannulae without further tissue manipulation for the correction of aging/thin skin in the periocular and perioral regions.

METHODS

Micro-superficial enhanced fluid fat injection (M-SEFFI) harvests adipose tissue with a multi-perforated cannula (0.3 mm), and autologous platelet rich plasma (PRP) is added. The tissue is injected into the dermal region of the periocular and perioral zones. Efficacy and viability were evaluated by histological and cell culture analysis. Clinical assessment included retrospective evaluation according to 1 = no effect, 2 = fair effect, 3 = good effect, 4 = excellent effect.

RESULTS

Between June 2014 and July 2015, 65 patients (7 men; mean age 49.7 years) were treated with M-SEFFI. No intraoperative complications or visible lumpiness were recorded. Analysis demonstrated mature, viable adipocytes with a strong stromal component. Following PRP addition, there was a greater proliferation noted in the M-SEFFI compared to the SEFFI (0.5 mm). Mean follow-up was 4.1 months. Clinical assessment by surgeons and patients at 1 month was 3.52 and 3.74, and 6 months 3.06 and 2.6 respectively.

CONCLUSIONS

M-SEFFI is effective and viable for lump free skin rejuvenation and volume enhancement, through the extraction of smoother ADSC rich, autologous fat tissue that does not require further tissue manipulation, to correct skin aging.

LEVEL OF EVIDENCE

4 Therapeutic.

摘要

背景

自体脂肪中的脂肪来源基质细胞和干细胞（ADSC）具有再生优势，注入真皮层和皮下层可促进皮肤年轻化并增加容积。然而，目前技术下极浅层脂肪注射存在局限性。

目的

评估使用极小侧孔（0.3毫米）套管采集脂肪且无需进一步组织处理来改善眼周和口周区域衰老/薄皮肤的效果及可行性。

方法

微浅表强化流体脂肪注射（M - SEFFI）使用多孔套管（0.3毫米）采集脂肪组织，并添加自体富血小板血浆（PRP）。将该组织注入眼周和口周区域的真皮层。通过组织学和细胞培养分析评估效果及可行性。临床评估包括根据1 = 无效果、2 = 一般效果、3 = 良好效果、4 = 极佳效果进行回顾性评估。

结果

2014年6月至2015年7月，65例患者（7名男性；平均年龄49.7岁）接受了M - SEFFI治疗。未记录到术中并发症或明显的肿块。分析显示存在成熟、有活力的脂肪细胞且基质成分丰富。添加PRP后，与SEFFI（0.5毫米）相比，M - SEFFI中细胞增殖更明显。平均随访4.1个月。外科医生和患者在1个月时的临床评估分别为3.52和3.74，6个月时分别为3.06和2.6。