Institute for Plastic Surgery, Southern Illinois University School of Medicine, Springfield, Illinois.
Aesthet Surg J. 2014 Jan 1;34(1):142-53. doi: 10.1177/1090820X13515700. Epub 2013 Dec 12.
Recent evidence suggests that lipofilling improves overlying skin composition and appearance. Adipose-derived stem cells (ADSC) have been implicated.
The authors identify ADSC transdifferentiation into epithelial stem cells through coexpression of GFP+ (green fluorescent protein positive) ADSC with the epithelial stem cell marker p63 in an in vivo fat grafting model.
Six male, GFP+ mice served as adipose tissue donors. Twelve nude mice served as recipients. Recipients were subdivided into 2 arms (6 mice/each arm) and received either whole-fat specimen (group 1) or isolated and purified ADSC + peptide hydrogel carrier (group 2) engrafted into a 1-cm(2) left parascapular subdermal plane. The right parascapular subdermal plane served as control. Skin flaps were harvested at 8 weeks and subjected to (1) confocal fluorescent microscopy and (2) reverse transcriptase polymerase chain reaction (RT-PCR) for p63 mRNA expression levels.
Gross examination of skin flaps demonstrated subjectively increased dermal vessel presence surrounding whole-fat and ADSC specimens. The GFP+ cells were seen within overlying dermal architecture after engraftment and were found to coexpress p63. Significantly increased levels of p63 expression were found in the ADSC + hydrogel skin flaps.
We offer suggestive evidence that GFP+ ADSC are found within the dermis 8 weeks after engraftment and coexpress the epithelial stem cell marker p63, indicating that ADSC may transdifferentiate into epithelial stem cells after fat grafting. These findings complement current understanding of how fat grafts may rejuvenate overlying skin.
最近的证据表明，脂肪填充术可以改善上层皮肤的成分和外观。脂肪来源的干细胞（ADSC）与此有关。
作者通过在体内脂肪移植模型中，共同表达 GFP+（绿色荧光蛋白阳性）ADSC 与上皮干细胞标志物 p63，鉴定 ADSC 向上皮干细胞的转分化。
6 只雄性 GFP+小鼠作为脂肪组织供体。12 只裸鼠作为受体。受体分为 2 组（每组 6 只），分别接受全脂标本（第 1 组）或分离和纯化的 ADSC+肽水凝胶载体（第 2 组）移植到 1cm²左侧肩胛下真皮下平面。右侧肩胛下真皮下平面作为对照。在 8 周时采集皮肤瓣，并进行（1）共聚焦荧光显微镜检查和（2）逆转录聚合酶链反应（RT-PCR）检测 p63 mRNA 表达水平。
皮肤瓣的大体检查显示，全脂和 ADSC 标本周围的真皮血管存在明显增加。移植后，在覆盖的真皮结构中可见 GFP+细胞，并发现其共表达 p63。ADSC+水凝胶皮肤瓣中 p63 表达水平显著增加。
我们提供了暗示性的证据，表明 GFP+ADSC 在移植后 8 周内可在真皮内发现，并共同表达上皮干细胞标志物 p63，表明 ADSC 在脂肪移植后可能向上皮干细胞转分化。这些发现补充了目前对脂肪移植如何使上层皮肤年轻化的理解。
