Li Tian, Wang Shimeng, Zhang Duo, Jiang Haiyue
Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P.R. China.
Department of Plastic and Reconstructive Surgery, The First Hospital of Jilin University, No.1, Xin Min Street, Chang Chun, Jilin, 130021, P.R. China.
Arch Dermatol Res. 2025 Feb 14;317(1):407. doi: 10.1007/s00403-025-03936-2.
To assess the efficacy of human adipose stem cells(ADSCs) in treating photo-aged skin using multi-needle injector versus syringe injection methods. Mice were divided into three groups: blank, model, and experimental. Skin photoaging was induced by eight weeks of UVB irradiation in the model and experimental groups. ADSCs (1 × 10 cells/120µL) were injected into the experimental group mice thrice over a 30-day period, using a syringe on one side and a multi-needle injector on the other side of the nude mice dorsum. Follow-up assessments one month post-injection included high-definition photography for wrinkle and needle mark analysis, histological evaluation of dermal thickness, collagen and elastic fibers. CD31 and Type I collagen levels were analyzed by immunohistochemistry, while MDA and SOD levels were assessed by ELISA assays. Post-UVB irradiation, significant photoaging signs were observed in the mice, including wrinkles and disordered collagen and elastic fibers. ADSCs treatment led to epidermal thinning, dermal collagen thickening, and improved collagen and elastic fiber organization. CD31 and Type I collagen expression increased, while MDA decreased, and SOD significantly increased. No significant differences were found between the injection methods. Multi-needle injector marks disappeared within 24 h, contrasting with syringe marks, and mass formation was reduced compared to syringe injections. ADSCs effectively ameliorate photoaging in mice. Multi-needle injector injections match the efficacy of syringe injections and offer advantages such as precise depth control and reduced mass formation, suggesting its potential as an effective tool for stem cell-based anti-aging treatments.
评估使用多针注射器与注射器注射方法将人脂肪干细胞（ADSCs）用于治疗光老化皮肤的疗效。将小鼠分为三组：空白组、模型组和实验组。模型组和实验组通过八周的紫外线B（UVB）照射诱导皮肤光老化。在30天内，将ADSCs（1×10个细胞/120µL）分三次注射到实验组小鼠体内，在裸鼠背部一侧使用注射器，另一侧使用多针注射器。注射后一个月的随访评估包括用于皱纹和针痕分析的高清摄影、真皮厚度、胶原蛋白和弹性纤维的组织学评估。通过免疫组织化学分析CD31和I型胶原蛋白水平，同时通过酶联免疫吸附测定（ELISA）评估丙二醛（MDA）和超氧化物歧化酶（SOD）水平。UVB照射后，在小鼠中观察到明显的光老化迹象，包括皱纹以及胶原蛋白和弹性纤维紊乱。ADSCs治疗导致表皮变薄、真皮胶原蛋白增厚，并改善了胶原蛋白和弹性纤维的排列。CD31和I型胶原蛋白表达增加，而MDA减少，SOD显著增加。两种注射方法之间未发现显著差异。与注射器痕迹相比，多针注射器痕迹在24小时内消失，并且与注射器注射相比，团块形成减少。ADSCs可有效改善小鼠的光老化。多针注射器注射与注射器注射的疗效相当，并具有诸如精确深度控制和减少团块形成等优点，表明其作为基于干细胞的抗衰老治疗有效工具的潜力。
