脂肪酸酰胺水解酶（FAAH）活性测定
Assay of FAAH Activity.
作者信息
Bari Monica, Feole Monica, Maccarrone Mauro
机构信息
Department of Experimental Medicine and Surgery, Tor Vergata University of Rome, Rome, Italy.
Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy.
出版信息
Methods Mol Biol. 2016;1412:131-6. doi: 10.1007/978-1-4939-3539-0_14.
Fatty acid amide hydrolase (FAAH) is an intracellular enzyme responsible for the hydrolysis of endogenous anandamide (AEA), a reaction that terminates the biological effects of this lipid mediator. The final products of this reaction are arachidonic acid and ethanolamine. In the method described herein, FAAH activity is measured through the use of a radioactive substrate by quantification of reaction products, that is, [(14)C]-ethanolamine from [(14)C-ethanolamine]-AEA.
脂肪酸酰胺水解酶（FAAH）是一种细胞内酶，负责内源性花生四烯乙醇胺（AEA）的水解，该反应终止了这种脂质介质的生物学效应。此反应的最终产物是花生四烯酸和乙醇胺。在本文所述的方法中，通过使用放射性底物对反应产物，即来自[(14)C - 乙醇胺]-AEA的[(14)C]-乙醇胺进行定量，来测量FAAH活性。
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