Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy.
Methods Mol Biol. 2023;2576:249-260. doi: 10.1007/978-1-0716-2728-0_21.
Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the degradation of anandamide (N-arachidonoylethanolamine, AEA) to arachidonic acid (AA) and ethanolamine. The method described here measures FAAH activity through the fluorometric arachidonoyl-7-amino-4-methyl-coumarin amide (AAMCA) substrate, which allows a simple and sensitive assay suitable for high-throughput screening tests. FAAH catalyzes the hydrolysis of AAMCA producing AA and the highly fluorescent compound 7-amino-4-methylcoumarin (AMC).
脂肪酸酰胺水解酶（FAAH）是负责降解花生四烯酸乙醇胺（N-花生四烯酸乙醇胺，AEA）生成花生四烯酸（AA）和乙醇胺的酶。这里描述的方法通过荧光素酰基-7-氨基-4-甲基香豆素酰胺（AAMCA）底物来测量 FAAH 的活性，这使得该方法成为一种简单灵敏的测定方法，适用于高通量筛选试验。FAAH 催化 AAMCA 的水解生成 AA 和高荧光化合物 7-氨基-4-甲基香豆素（AMC）。
