放射分析法测定 FAAH 活性。

Radiometric Assay of FAAH Activity.

机构信息

Department of Experimental Medicine, Tor Vergata University of Rome, Rome, Italy.

European Center for Brain Research/Santa Lucia Foundation IRCCS, Rome, Italy.

出版信息

Methods Mol Biol. 2023;2576:241-247. doi: 10.1007/978-1-0716-2728-0_20.

DOI:10.1007/978-1-0716-2728-0_20

PMID:36152192

Abstract

Fatty acid amide hydrolase (FAAH) is an intracellular enzyme responsible for the hydrolysis of endogenous anandamide (AEA), a reaction that terminates the biological effects of this lipid mediator. The final products of AEA cleavage are arachidonic acid and ethanolamine. In the method described herein, FAAH activity is measured through the use of the radioactive substrate [C-ethanolamine]-AEA and subsequent quantification of the reaction product [C]-ethanolamine.

摘要

脂肪酸酰胺水解酶（FAAH）是一种细胞内酶，负责水解内源性大麻素（AEA），这一反应终止了这种脂类介质的生物学效应。AEA 裂解的终产物是花生四烯酸和乙醇胺。在本文描述的方法中，通过使用放射性底物 [C-乙醇胺]-AEA 并随后定量反应产物 [C]-乙醇胺来测量 FAAH 活性。