Hu Ruiqi, Xi Liqin, Cao Qing, Yang Rui, Liu Yuning, Sheng Xia, Han Yingying, Yuan Zhengrong, Guo Yan, Weng Qiang, Xu Meiyu
College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
College of Basic Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, PR China.
Prostaglandins Other Lipid Mediat. 2016 Jul;124:9-15. doi: 10.1016/j.prostaglandins.2016.05.006. Epub 2016 May 28.
The Chinese brown frog (Rana dybowskii) has one special physiological phenomenon, which is that its oviduct expands prior to hibernation rather than in the breeding period. In this study, we investigated the immunolocalization and expression levels of prostaglandin-E2 (PGE2), cyclooxygenase (COX)-1 and COX-2, as well as one of its receptor subtypes 4 (EP4) in the oviduct of Rana dybowskii during the pre-hibernation and breeding period. PGE2, COX-1, COX-2 and EP4 have been observed in glandular and epithelial cells in the breeding period, whereas only in the epithelial cells during the pre-hibernation. Consistently, the protein levels of COX-2 and EP4 were higher in the pre-hibernation as compared to the breeding period, but the diversity of COX-1 was not obvious. In addition, oviductal PGE2 concentration was also significantly higher in the pre-hibernation. These results suggested that prostaglandin-E2 may play an important autocrine or paracrine role in oviductal cell proliferation and differentiation of Rana dybowskii during pre-hibernation.
中国林蛙（东北林蛙）有一种特殊的生理现象，即其输卵管在冬眠前而非繁殖期扩张。在本研究中，我们调查了冬眠前和繁殖期中国林蛙输卵管中前列腺素 - E2（PGE2）、环氧化酶（COX）-1和COX-2及其受体亚型之一EP4的免疫定位和表达水平。在繁殖期，PGE2、COX-1、COX-2和EP4在腺细胞和上皮细胞中均有观察到，而在冬眠前仅在上皮细胞中存在。一致的是，与繁殖期相比，冬眠前COX-2和EP4的蛋白水平更高，但COX-1的差异不明显。此外，冬眠前输卵管PGE2浓度也显著更高。这些结果表明，前列腺素 - E2可能在冬眠前中国林蛙输卵管细胞增殖和分化中发挥重要的自分泌或旁分泌作用。
