Singh Akanand, Yang Kang, Liu Zuojin, Gong Jianping
Chongqing Key Laboratory of Hepatobiliary Surgery and Department of Hepatobiliary Surgery, The Second Affiliated Hospital, Chongqing Medical University, No. 76 Linjiang Road, Yuzhong District, Chongqing, 400010 People's Republic of China.
Springerplus. 2016 May 12;5:606. doi: 10.1186/s40064-016-2222-5. eCollection 2016.
Acute obstructive cholangitis (AOC) is a severe disease that leads to sepsis, shock and multiple organ dysfunction syndrome. Liver is the largest and the major organ for the defense mechanisms during the sepsis. Our aim was to investigate the expression of scavenger receptor A (SRA) in rat's liver tissue during AOC and its relation with the inflammatory mediators and hepatic injury caused by endotoxins.
Ninety Wistar rats were randomly divided into three equal groups. In group one, the choledochus were ligagted and Escherichia coli O111B4 injected into it to produce AOC model. Group two had choledochus ligated and group three had sham surgery. Six animals from each group were sacrificed at 0, 3, 6, 12 and 24 h for comparative analysis of endotoxins, tumor necrosis factor-alpha, interleukin-6 in plasma and expression of SRA protein and SRA mRNA in liver tissue. The P < 0.05 was considered significant. Ethical approval was obtained.
The plasma endotoxins, tumor necrosis factor-alpha and interleukin-6 levels in AOC rats increased progressively with time. The SRA protein and SRA mRNA expression decreased with time. The differences among groups were significant (P < 0.01). Liver histopathology showed gradually aggravated hepatic injury with neutrophil infiltration, degeneration and necrosis of hepatocytes.
In AOC, the expression of SRA in liver gradually decreased with progressive increase in level of plasma endotoxins and hepatic injury suggesting its importance in the defense mechanism.
急性梗阻性胆管炎（AOC）是一种严重疾病，可导致脓毒症、休克及多器官功能障碍综合征。肝脏是脓毒症期间防御机制中最大且主要的器官。我们的目的是研究急性梗阻性胆管炎期间大鼠肝脏组织中清道夫受体A（SRA）的表达及其与内毒素所致炎症介质和肝损伤的关系。
将90只Wistar大鼠随机分为三组，每组30只。第一组结扎胆总管并注入大肠杆菌O111B4以建立急性梗阻性胆管炎模型；第二组仅结扎胆总管；第三组行假手术。每组分别于0、3、6、12和24小时处死6只动物，用于比较分析血浆中内毒素、肿瘤坏死因子-α、白细胞介素-6水平以及肝脏组织中SRA蛋白和SRA mRNA的表达。P<0.05为差异有统计学意义。本研究获得了伦理批准。
急性梗阻性胆管炎大鼠血浆内毒素、肿瘤坏死因子-α和白细胞介素-6水平随时间逐渐升高。SRA蛋白和SRA mRNA表达随时间下降。组间差异有统计学意义（P<0.01）。肝脏组织病理学显示肝损伤逐渐加重，伴有中性粒细胞浸润、肝细胞变性和坏死。
在急性梗阻性胆管炎中，随着血浆内毒素水平和肝损伤的逐渐加重，肝脏中SRA的表达逐渐降低，提示其在防御机制中的重要性。
