Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey.
Sabanci University Nanotechnology Research and Application Center, Orta Mah., 34956 Istanbul, Turkey.
Colloids Surf B Biointerfaces. 2016 Oct 1;146:39-46. doi: 10.1016/j.colsurfb.2016.05.053. Epub 2016 May 18.
In this study, soil bacteria were isolated from nanomaterials (NMs) contaminated pond soil and enriched in the presence of graphene oxide (GO) in mineral medium to obtain NMs resistant bacteria. The isolated resistant bacteria were biochemically and genetically identified as Fontibacillus aquaticus. The resistant bacteria were allowed to interact with engineered GO in order to study the biotransformation in GO structure. Raman spectra of GO extracted from culture medium revealed decreased intensity ratio of ID/IG with subsequent reduction of CO which was consistent with Fourier transform infrared (FTIR) results. The structural changes and exfoliatied GO nanosheets were also evident from transmission electron microscopy (TEM) images. Ultraviolet-visible spectroscopy, high resolution X-ray diffraction (XRD) and current-voltage measurements confirmed the reduction of GO after the interaction with resistant bacteria. X-ray photoelectron spectroscopy (XPS) analysis of biotransformed GO revealed reduction of oxygen-containing species on the surface of nanosheets. Our results demonstrated that the presented method is an environment friendly, cost effective, simple and based on green approaches for the reduction of GO using NMs resistant bacteria.
在这项研究中，从纳米材料（NMs）污染的池塘土壤中分离出土壤细菌，并在含有氧化石墨烯（GO）的矿物培养基中进行富集，以获得对 NMs 具有抗性的细菌。分离出的抗性细菌通过生物化学和基因鉴定为水生 Fontibacillus 细菌。让抗性细菌与工程 GO 相互作用，以研究 GO 结构中的生物转化。从培养基中提取的 GO 的拉曼光谱显示 ID/IG 的强度比随着随后 CO 的减少而降低，这与傅里叶变换红外（FTIR）的结果一致。结构变化和 GO 纳米片的剥落也可以从透射电子显微镜（TEM）图像中看出。紫外-可见光谱、高分辨率 X 射线衍射（XRD）和电流-电压测量证实了与抗性细菌相互作用后 GO 的还原。生物转化的 GO 的 X 射线光电子能谱（XPS）分析表明，纳米片表面的含氧物种减少。我们的结果表明，所提出的方法是一种环境友好、经济高效、简单且基于绿色方法的利用 NMs 抗性细菌还原 GO 的方法。
