Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China.
Sci Rep. 2019 Aug 26;9(1):12376. doi: 10.1038/s41598-019-48904-9.
The effect of a 2-aminoanthraquinone-graphene oxide (AQ-GO) composite on the anaerobic dechlorination and degradation of chloroanilines by an enriched bacterial consortium was investigated. The results showed that the maximal degradation efficiency of 20 mg/L 2-chloroaniline (2-CA) reached 91.4% at a dose of 20 mg/L AQ-GO in 30 d. Moreover, the pseudo-first-order rate constant of 2-CA degradation in the AQ-GO-mediated system was 2.9-fold higher than those in AQ- and GO-mediated systems alone. During this process, a synergetic effect between AQ and GO was observed, which was attributed to the increased intracellular and extracellular electron transfer pathways. GC-MS analysis showed that 2-CA could be degraded to hexanoic acid and ultimately mineralized to CO. Illumina MiSeq sequencing revealed that additional AQ-GO significantly increased the relative abundance of Firmicutes. Further analysis showed that the populations of the genera Oscillospira, unclassified Lactobacillales, unclassified Veillonellaceae and Ruminococcus exhibited positive correlations with the rate constant of 2-CA degradation and the dehydrogenase activity of bacterial consortium. These findings indicated that AQ-GO promoted the enrichment of functional bacteria and increased the bacterial activity, resulting in the enhanced dechlorination and degradation of 2-chloroaniline.
研究了 2-氨基蒽醌-氧化石墨烯（AQ-GO）复合材料对富集细菌共混物厌氧脱氯和降解氯代苯胺的影响。结果表明，在 30d 时，20mg/L AQ-GO 的剂量下，20mg/L 2-氯苯胺（2-CA）的最大降解效率达到 91.4%。此外，在 AQ-GO 介导的体系中，2-CA 降解的拟一级速率常数比 AQ 和 GO 单独介导的体系高 2.9 倍。在这个过程中，观察到 AQ 和 GO 之间存在协同效应，这归因于增加了细胞内和细胞外的电子传递途径。GC-MS 分析表明，2-CA 可降解为己酸，并最终矿化为 CO。Illumina MiSeq 测序表明，额外的 AQ-GO 显著增加了厚壁菌门的相对丰度。进一步分析表明，属 Oscillospira、未分类的乳杆菌目、未分类的韦荣氏球菌科和瘤胃球菌属的种群与 2-CA 降解的速率常数和细菌共混物的脱氢酶活性呈正相关。这些发现表明，AQ-GO 促进了功能细菌的富集，并增加了细菌的活性，从而增强了 2-氯苯胺的脱氯和降解。
