Bioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 3052, Australia.
Department of Medical Biology, University of Melbourne, Melbourne, VIC, 3010, Australia.
Gigascience. 2016 Jun 2;5(1):23. doi: 10.1186/s13742-016-0129-2.
The scabies mite, Sarcoptes scabiei, is a parasitic arachnid and cause of the infectious skin disease scabies in humans and mange in other animal species. Scabies infections are a major health problem, particularly in remote Indigenous communities in Australia, where secondary group A streptococcal and Staphylococcus aureus infections of scabies sores are thought to drive the high rate of rheumatic heart disease and chronic kidney disease.
We sequenced the genome of two samples of Sarcoptes scabiei var. hominis obtained from unrelated patients with crusted scabies located in different parts of northern Australia using the Illumina HiSeq. We also sequenced samples of Sarcoptes scabiei var. suis from a pig model. Because of the small size of the scabies mite, these data are derived from pools of thousands of mites and are metagenomic, including host and microbiome DNA. We performed cleaning and de novo assembly and present Sarcoptes scabiei var. hominis and var. suis draft reference genomes. We have constructed a preliminary annotation of this reference comprising 13,226 putative coding sequences based on sequence similarity to known proteins.
We have developed extensive genomic resources for the scabies mite, including reference genomes and a preliminary annotation.
疥螨，是一种寄生性节肢动物，也是导致人类传染性皮肤病疥疮和其他动物物种的螨病的病原体。疥疮感染是一个主要的健康问题，特别是在澳大利亚偏远的土著社区，那里的疥疮疮继发 A 组链球菌和金黄色葡萄球菌感染被认为是导致风湿性心脏病和慢性肾病高发的原因。
我们使用 Illumina HiSeq 对来自澳大利亚北部不同地区的两名患有结痂性疥疮的无关患者的两种人型疥螨样本进行了测序。我们还对来自猪模型的猪型疥螨样本进行了测序。由于疥螨体型较小，这些数据来自数千只螨虫的混合样本，属于宏基因组学，包括宿主和微生物组的 DNA。我们进行了清理和从头组装，呈现了人型疥螨和猪型疥螨的参考基因组草案。我们构建了一个初步的注释，基于与已知蛋白的序列相似性，该注释包含了 13226 个假定的编码序列。
我们已经为疥螨开发了广泛的基因组资源，包括参考基因组和初步注释。
