Shinohara Yukiya, Nakamura Toshiaki, Shirakata Yoshinori, Noguchi Kazuyuki
Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences.
Dent Mater J. 2016;35(3):454-60. doi: 10.4012/dmj.2015-242.
The aim of this study was to examine the effects of recombinant human BMP-9 (rhBMP-9) with chitosan sponge (ChiS) or absorbable collagen sponge (ACS) on bone formation in rat calvarial defects. The defects were treated by one of the following implantations: ChiS, rhBMP-9/ChiS, ACS, rhBMP-9/ACS and no implantation. The animals were euthanized at 8 weeks for histological evaluation. The percentage of defect closure (DC) in the rhBMP-9/ACS group was significantly greater than that in the ACS group. The rhBMP-9/ACS group demonstrated the highest level of DC among all the groups. The newly formed bone area (NBA) and NBA/total area in the ChiS-implanted groups and in the rhBMP-9/ACS group were significantly greater compared with those in the ACS group. It can be concluded that rhBMP-9/ACS has a potential to induce bone formation in rat calvarial defects. Further studies are required to elucidate the mechanism of bone formation induced by rhBMP-9.
本研究的目的是检测重组人骨形态发生蛋白-9（rhBMP-9）与壳聚糖海绵（ChiS）或可吸收胶原海绵（ACS）对大鼠颅骨缺损骨形成的影响。通过以下植入方式之一治疗缺损：ChiS、rhBMP-9/ChiS、ACS、rhBMP-9/ACS以及不植入。8周时对动物实施安乐死以进行组织学评估。rhBMP-9/ACS组的缺损闭合（DC）百分比显著高于ACS组。rhBMP-9/ACS组在所有组中DC水平最高。与ACS组相比，植入ChiS的组和rhBMP-9/ACS组的新形成骨面积（NBA）以及NBA/总面积显著更大。可以得出结论，rhBMP-9/ACS有诱导大鼠颅骨缺损骨形成的潜力。需要进一步研究以阐明rhBMP-9诱导骨形成的机制。
