Kondo Hiroki, Takayama Tadahiro, Onizawa Takashi, Isobe Shunsuke, Tanabe Natsuko, Suzuki Naoto, Yamano Seiichi, Sato Shuichi
Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry, Tokyo, Japan.
Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan.
J Dent Sci. 2024 Oct;19(4):2114-2125. doi: 10.1016/j.jds.2024.04.004. Epub 2024 Apr 17.
BACKGROUND/PURPOSE: Bone morphogenetic protein-9 (BMP-9) has demonstrated multiple advantages in promoting osteogenesis. Our previous findings have indicated that the use of an absorbable collagen membrane (ACM) as a carrier for growth factors is effective in stimulating bone regeneration. The objective of this study was to assess the synergistic impact of BMP-9 incorporated into ACM (ACM/BMP-9) on bone formation within rat mandibular bone defects.
Circular bone defects of critical size were surgically induced on both sides of the rat mandibular bone, with subsequent random allocation into distinct groups: control, ACM alone, and ACM loaded with low (0.5 μg) or high (2.0 μg) concentrations of BMP-9. We conducted real-time in vivo micro-computerized tomography scans at the baseline and at 2, 4, and 6 weeks, and measured the volume of newly formed bone (NFB), bone mineral density (BMD) of NFB, and the closure percentage of the NFB area. Histological and histomorphometric analyses were performed at 6 weeks.
Real-time assessment revealed notably higher levels of bone volume, BMD, and closure percentage in the NFB area for the groups treated with ACM/BMP-9 compared to the control and ACM groups. Within the high concentration of BMP-9 group, the volume and BMD of NFB exhibited a significant increase at 6 weeks compared to baseline. Histological examination confirmed the existence of osteoblasts, osteocytes, and blood vessels within the NFB.
Considering the limitations of this research, the real-time evaluation finding indicates that ACM/BMP-9 effectively promotes bone formation in critical-size mandibular defects in rats.
背景/目的：骨形态发生蛋白-9（BMP-9）在促进成骨方面已展现出多种优势。我们之前的研究结果表明，使用可吸收胶原膜（ACM）作为生长因子的载体可有效刺激骨再生。本研究的目的是评估掺入ACM的BMP-9（ACM/BMP-9）对大鼠下颌骨缺损内骨形成的协同影响。
在大鼠下颌骨两侧手术制造临界尺寸的圆形骨缺损，随后随机分为不同组：对照组、单纯ACM组以及负载低浓度（0.5μg）或高浓度（2.0μg）BMP-9的ACM组。在基线以及第2、4和6周进行实时体内微型计算机断层扫描，并测量新形成骨（NFB）的体积、NFB的骨密度（BMD）以及NFB区域的闭合百分比。在第6周进行组织学和组织形态计量学分析。
实时评估显示，与对照组和ACM组相比，ACM/BMP-9处理组的NFB区域骨体积、BMD和闭合百分比水平显著更高。在高浓度BMP-9组中，与基线相比，NFB的体积和BMD在第6周时显著增加。组织学检查证实NFB内存在成骨细胞、骨细胞和血管。
考虑到本研究的局限性，实时评估结果表明ACM/BMP-9可有效促进大鼠临界尺寸下颌骨缺损的骨形成。
