Analytical Toxicology and Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Analytical Toxicology and Mineral Metabolism Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Arch Med Res. 2016 Apr;47(3):232-5. doi: 10.1016/j.arcmed.2016.05.005. Epub 2016 May 31.
Increased levels of metallothionein (MT) have recently been found in the blood serum of men with newly diagnosed testicular germ cell tumors (TGCT). In light of previously published results, the aim of this study was to investigate the difference in serum MT levels among patients with different stages of TGCT and compare MT with commonly used markers (α-fetoprotein, β-human chorionic gonadotropin and lactate dehydrogenase). The concentration of total MT was determined in the serum of 25 men with TGCT (seminoma or non-seminoma) by differential pulse voltammetry. Serum samples were obtained prior to chemotherapy, after two cycles of chemotherapy and 1 year after chemotherapy. A statistically significant difference in MT levels in patients with different stages of TGCT was observed in the serum of patients with non-seminoma obtained before chemotherapy. Although not significant, an increase in serum MT levels commensurate with the disease stage increase was also observed in patients with seminomatous TGCT. The results indicate that, in combination with the existing markers, MT could be useful for the identification of the histological type of tumor and stage of the disease before biopsy diagnosis.
最近在新诊断的睾丸生殖细胞肿瘤 (TGCT) 男性的血清中发现金属硫蛋白 (MT) 水平升高。鉴于之前发表的结果，本研究旨在研究不同分期 TGCT 患者血清 MT 水平的差异，并将 MT 与常用标志物（甲胎蛋白、β-人绒毛膜促性腺激素和乳酸脱氢酶）进行比较。通过差分脉冲伏安法测定 25 例 TGCT（精原细胞瘤或非精原细胞瘤）男性患者血清中的总 MT 浓度。在化疗前、化疗 2 个周期后和化疗后 1 年采集血清样本。在化疗前非精原细胞瘤患者的血清中观察到不同分期 TGCT 患者 MT 水平存在统计学显著差异。虽然不显著，但在精原细胞瘤患者中也观察到与疾病阶段增加相匹配的血清 MT 水平升高。这些结果表明，MT 与现有的标志物结合使用，可在活检诊断前用于识别肿瘤的组织学类型和疾病分期。
