[苏云金芽孢杆菌的种内质粒转化]
[Intraspecies plasmid transformation of Bacillus thuringiensis].
作者信息
Bessol'tsev V V, Zhuchilin L I
出版信息
Mol Gen Mikrobiol Virusol. 1989 Mar(3):25-8.
PMID:2725537
Abstract
Bacillus thuringiensis strains 1009, 3004, 3029 of the natural isolates of var. dendrolimus harbouring two large plasmids were transformed by a mix of small plasmids DNA. One of transforming plasmids carried a gene for tetracyclinresistance. The natural isolates of var. dendrolimus strain 2002 and var. berliner strain 1035 were used as donors for plasmid DNA transformation. Three variants of plasmid spectra registered in tetracycliresistant transformants are discussed.
摘要
携带两个大质粒的苏云金芽孢杆菌松毛虫变种的天然分离株1009、3004、3029，用小质粒DNA混合物进行转化。其中一个转化质粒携带四环素抗性基因。松毛虫变种2002的天然分离株和库斯塔克变种1035用作质粒DNA转化的供体。讨论了在四环素抗性转化体中登记的三种质粒谱变体。
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