Mafi Afsaneh, Dehghani Farzaneh, Moghadam Abbas, Noorafshan Ali, Vojdani Zahra, Talaei-Khozani Tahereh
a Anatomy Department , Shiraz University of Medical Sciences , Shiraz , Iran.
b Anatomy Department , Stereology and Histomorphology Research Center, Shiraz University of Medical Sciences , Shiraz , Iran.
Platelets. 2016 Dec;27(8):771-776. doi: 10.1080/09537104.2016.1184749. Epub 2016 Jun 2.
Numerous bioactive growth factors and cytokines in platelet-rich plasma (PRP) have recently made it an attractive biomaterial for therapeutic purposes. These growth factors have the potential to regenerate the injured tissues. The aim of this study was to investigate the therapeutic effects of PRP in hepatotoxic animal model. Hepatotoxicity was induced in rats by oral administration of 4 mL/kg/week of CCl diluted 1:1 in corn oil for 10 weeks. To confirm the hepatotoxicity, 24 h after the last CCl administration, blood samples were collected via cardiac puncture to assess the serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, albumin, total protein, and total bilirubin. Twenty-four hours after blood collection, the experimental animals received a single injection of PRP (1 mL) via the anterior mesenteric vein. One week later, all biochemical tests were performed again, and the rats were scarified and their livers were removed, prepared histologically, and stained. The stereological analyses were performed to evaluate the effects of PRP on histopathological features of CCl-treated livers. The results were compared statistically with the corresponding control and CCl+normal saline (NS)-treated animals. A significant decrease in the number and volume of hepatocytes (p = 0.01), and also a reduction in the volume of sinusoids (p = 0.001) and connective tissue (p = 0.04), were observed in the PRP-treated animals compared with the CCl+NS-treated ones. Our findings demonstrated that application of PRP had beneficial effects on CCl-induced fibrosis; however, it had detrimental effects on the total number of hepatocytes and the volume of hepatocytes and sinusoidal spaces.
富含血小板血浆（PRP）中的多种生物活性生长因子和细胞因子，近来使其成为一种用于治疗目的的有吸引力的生物材料。这些生长因子有使受损组织再生的潜力。本研究的目的是调查PRP在肝毒性动物模型中的治疗效果。通过每周经口给予1:1稀释于玉米油中的四氯化碳（CCl）4 mL/kg，持续10周，诱导大鼠产生肝毒性。为确认肝毒性，在最后一次给予CCl后24小时，通过心脏穿刺采集血样，以评估血清中丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶、白蛋白、总蛋白和总胆红素的水平。采血24小时后，实验动物经肠系膜前静脉接受单次PRP注射（1 mL）。一周后，再次进行所有生化检测，然后处死大鼠，取出肝脏，进行组织学制备和染色。进行体视学分析，以评估PRP对CCl处理肝脏组织病理学特征的影响。将结果与相应的对照组和CCl+生理盐水（NS）处理的动物进行统计学比较。与CCl+NS处理的动物相比，PRP处理的动物肝细胞数量和体积显著减少（p = 0.01），并且肝血窦体积（p = 0.001）和结缔组织体积（p = 0.04）也有所减少。我们的研究结果表明，应用PRP对CCl诱导的纤维化有有益作用；然而，它对肝细胞总数、肝细胞体积和肝血窦空间有不利影响。
