Du Zhenguo, Lin Wenzhong, Qiu Ping, Liu Xiaojuan, Guo Lingfang, Wu Kangcheng, Zhang Songbai, Wu Zujian
Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.
Arch Virol. 2016 Aug;161(8):2343-6. doi: 10.1007/s00705-016-2911-y. Epub 2016 Jun 2.
A double-stranded RNA (dsRNA) HBJZ1506 recovered from the phytopathogenic fungus Erysiphe cichoracearum infecting Calendula officinalis in Jingzhou, Hubei Province, China, was sequenced. HBJZ1506 comprises 11,908 nucleotides (nt) and contains a 11,859-nt-long open reading frame (ORF) coding for a polypeptide that is 61 % identical to that of a putative endornavirus named grapevine endophyte endornavirus (GeEV). The putative polyprotein has an RNA-dependent RNA polymerase (RdRp) domain and an RNA helicase domain, which show homology to and have an arrangement that is similar to that of their counterparts in approved or putative endornaviruses. In a phylogenetic tree constructed using amino acid sequences of the RdRp region of HBJZ1506 and selected endornaviruses, HBJZ1506 clustered with endornaviruses and formed a well-supported monophyletic branch with GeEV. These results suggest that HBJZ1506 might represent a novel endornavirus, for which the name Erysiphe cichoracearum endornavirus (EcEV) is proposed.
从中国湖北省荆州市感染金盏花的植物致病真菌菊科白粉菌中分离出一种双链RNA(dsRNA)HBJZ1506,并对其进行了测序。HBJZ1506由11908个核苷酸(nt)组成,包含一个11859 nt长的开放阅读框(ORF),编码一种与假定的葡萄内生病毒葡萄藤内生病毒(GeEV)的多肽具有61%同一性的多肽。假定的多蛋白具有一个RNA依赖性RNA聚合酶(RdRp)结构域和一个RNA解旋酶结构域,它们与已批准或假定的内病毒中的对应结构域具有同源性且排列相似。在使用HBJZ1506的RdRp区域氨基酸序列和选定的内病毒构建的系统发育树中,HBJZ1506与内病毒聚集在一起,并与GeEV形成了一个得到充分支持的单系分支。这些结果表明,HBJZ1506可能代表一种新型内病毒,为此提出了菊科白粉菌内病毒(EcEV)这一名称。