School of Biological Sciences, The University of Auckland, PO Box 92019, Auckland 1010, New Zealand.
School of Biological Sciences, The University of Auckland, PO Box 92019, Auckland 1010, New Zealand.
Virus Res. 2014 Aug 30;189:303-9. doi: 10.1016/j.virusres.2014.06.010. Epub 2014 Jun 28.
The complete sequence and genome organisation of an endornavirus from the phytopathogenic fungus Sclerotinia sclerotiorum isolate 11691 was described and the name Sclerotinia sclerotiorum endornavirus 1 (SsEV1/11691) proposed. The genome is 10,513 nucleotides (nts) long with a single open reading frame (ORF) that codes for a single polyprotein of 3459 amino acid (aa) residues. The polyprotein contains cysteine-rich region (CRR), viral methyltransferase (MTR), putative DEXDc, viral helicase (Hel), phytoreo_S7 (S7) and RNA-dependent RNA polymerase (RdRp) domains. The polyprotein and the conserved domains are phylogenetically related to endornaviruses. However, the coding strand of SsEV1/11691 does not contain a site-specific nick characteristic of most previously described endornaviruses. The elimination of SsEV1/11691 did not result in any significant changes in the host phenotype and virulence.
本文描述了一种来自植物病原菌核盘菌分离株 11691 的内源性病毒的完整序列和基因组结构，并提出了名称为核盘菌内源性病毒 1（SsEV1/11691）。该基因组长 10513 个核苷酸（nt），具有单一的开放阅读框（ORF），编码一个由 3459 个氨基酸（aa）残基组成的单一多蛋白。多蛋白包含富含半胱氨酸的区域（CRR）、病毒甲基转移酶（MTR）、假定的 DEXDc、病毒解旋酶（Hel）、phytoreo_S7（S7）和 RNA 依赖性 RNA 聚合酶（RdRp）结构域。多蛋白和保守结构域与内源性病毒在系统发育上具有相关性。然而，SsEV1/11691 的编码链并不包含大多数先前描述的内源性病毒所具有的特异性位点缺口。消除 SsEV1/11691 并不会导致宿主表型和毒力发生任何显著变化。
