来自植物病原真菌灰葡萄孢的一种新型内质网病毒的分子特征分析
Molecular characterization of a novel endornavirus from the phytopathogenic fungus Botrytis cinerea.
作者信息
Hao Fangmin, Zhou Ziliang, Wu Mingde, Li Guoqing
机构信息
The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
出版信息
Arch Virol. 2017 Jan;162(1):313-316. doi: 10.1007/s00705-016-3106-2. Epub 2016 Oct 8.
The complete sequence of a novel endornavirus (Botrytis cinerea endornavirus 1, BcEV1) from the phytopathogenic fungus Botrytis cinerea strain HBtom-372 was determined. The BcEV1 coding strand is 11,557 nucleotides long, possessing an open reading frame (ORF) that codes for a polyprotein of 3,787 amino acid residues and lacks a site-specific nick. The polyprotein contains viral methyltransferase (MTR) domain, a cysteine-rich region (CRR), two putative viral helicase (DEXDc-like and Hel-1) domains, and an RNA-dependent RNA polymerase_2 (RdRp_2) domain. In phylogenetic analysis, BcEV1 clustered with several fungal endornaviruses, forming an independent clade, and it was detected in 4.2 % of B. cinerea strains collected from central China.
测定了来自植物病原真菌灰葡萄孢菌HBtom - 372株系的一种新型内质网病毒（灰葡萄孢菌内质网病毒1，BcEV1）的完整序列。BcEV1编码链长11,557个核苷酸，具有一个开放阅读框（ORF），该阅读框编码一个由3,787个氨基酸残基组成的多蛋白，并且没有位点特异性切口。该多蛋白包含病毒甲基转移酶（MTR）结构域、富含半胱氨酸区域（CRR）、两个假定的病毒解旋酶（DEXDc样和Hel - 1）结构域以及一个RNA依赖的RNA聚合酶_2（RdRp_2）结构域。在系统发育分析中，BcEV1与几种真菌内质网病毒聚类，形成一个独立的进化枝，并且在从中国中部收集的4.2%的灰葡萄孢菌菌株中检测到该病毒。
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