Bangani Nonzwakazi, Nakiwala Justine, Martineau Adrian R, Wilkinson Robert J, Wilkinson Katalin A, Lowe David M
*Clinical Infectious Diseases Research Initiative, Institute for Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa; †Institute of Tropical Medicine, Antwerp, Belgium; ‡Medical Research Council (MRC)/Uganda Virus Research Institute (UVRI) Uganda Research Unit on AIDS, Entebbe, Uganda; §Blizard Institute, Barts and The London School of Medicine, Queen Mary University of London, London, United Kingdom; ‖Department of Medicine, Imperial College London, London, United Kingdom; ¶Mill Hill Laboratory, The Francis Crick Institute, London, United Kingdom; and #Institute of Immunity and Transplantation, University College London, London, United Kingdom.
J Acquir Immune Defic Syndr. 2016 Nov 1;73(3):263-267. doi: 10.1097/QAI.0000000000001103.
Using a flow cytometric assay, we investigated neutrophil-Mycobacterium tuberculosis opsonophagocytosis and the impact of HIV-1-infected serum on this process. The mean (±SD) percentage of neutrophils internalizing bacilli after 30 minutes incubation was significantly reduced by pretreatment with anti-CD16 (18.2% ± 8.1%, P < 0.001) or anti-CD35 antibody (23.2% ± 10.6%, P < 0.05) versus anti-CD4 controls (29.9% ± 8.1%). Blocking CD88 or CD11a did not affect internalization. Using heat-inactivated serum, maximal internalization was lower using HIV-1-infected serum versus HIV-1-uninfected. Using non-heat-inactivated serum, internalization decreased more rapidly with sequential dilutions of HIV-1-infected versus HIV-1-uninfected serum. CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis.
我们采用流式细胞术检测法，研究了中性粒细胞对结核分枝杆菌的调理吞噬作用以及HIV-1感染血清对该过程的影响。与抗CD4对照（29.9%±8.1%）相比，用抗CD16（18.2%±8.1%，P<0.001）或抗CD35抗体（23.2%±10.6%，P<0.05）预处理后，孵育30分钟内化杆菌的中性粒细胞的平均（±标准差）百分比显著降低。阻断CD88或CD11a不影响内化。使用热灭活血清时，与未感染HIV-1的血清相比，感染HIV-1的血清的最大内化率较低。使用非热灭活血清时，随着HIV-1感染血清与未感染HIV-1血清的连续稀释，内化作用下降得更快。CD16和CD35对中性粒细胞内化结核分枝杆菌很重要，而HIV-1感染会对调理吞噬作用产生不利影响。
