School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, PR China; Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, PR China.
School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, PR China.
Environ Int. 2016 Sep;94:161-169. doi: 10.1016/j.envint.2016.05.021. Epub 2016 May 31.
Emission of polycyclic aromatic hydrocarbons (PAHs) from e-waste recycling activities in China is known. However, little is known on the association between PAH exposure and oxidative damage to DNA and lipid content in people living near e-waste dismantling sites. In this study, ten hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) and two biomarkers [8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA)] of oxidative stress were investigated in urine samples collected from people living in and around e-waste dismantling facilities, and in reference population from rural and urban areas in China. The urinary levels of ∑10OH-PAHs determined in e-waste recycling area (GM: 25.4μg/g Cre) were significantly higher (p<0.05) than those found in both rural (11.7μg/g Cre) and urban (10.9μg/g Cre) reference areas. The occupationally exposed e-waste workers (36.6μg/g Cre) showed significantly higher (p<0.01) urinary Σ10OH-PAHs concentrations than non-occupationally exposed people (23.2μg/g Cre) living in the e-waste recycling site. The differences in urinary Σ10OH-PAHs levels between smokers (23.4μg/g Cre) and non-smokers (24.7μg/g Cre) were not significant (p>0.05) in e-waste dismantling sites, while these differences were significant (p<0.05) in rural and urban reference areas; this indicated that smoking is not associated with elevated levels of PAH exposure in e-waste dismantling site. Furthermore, we found that urinary concentrations of Σ10OH-PAHs and individual OH-PAHs were significantly associated with elevated 8-OHdG, in samples collected from e-waste dismantling site; the levels of urinary 1-hydroxypyrene (1-PYR) (r=0.284, p<0.01) was significantly positively associated with MDA. Our results indicate that the exposure to PAHs at the e-waste dismantling site may have an effect on oxidative damage to DNA among selected participants, but this needs to be validated in large studies.
已知中国电子废物回收活动会排放多环芳烃（PAHs）。然而，人们对生活在电子废物拆解场附近的人接触多环芳烃与 DNA 氧化损伤和脂质含量之间的关系知之甚少。在这项研究中，我们调查了生活在电子废物拆解设施内和周围以及中国农村和城市参考人群的尿液样本中 10 种羟基多环芳烃（OH-PAHs）和 2 种氧化应激生物标志物[8-羟基-2'-脱氧鸟苷（8-OHdG）和丙二醛（MDA）]的水平。电子废物回收区（25.4μg/g Cre）的尿液中∑10OH-PAHs 含量明显高于农村（11.7μg/g Cre）和城市（10.9μg/g Cre）参考区。职业接触电子废物的工人（36.6μg/g Cre）的尿液中∑10OH-PAHs 浓度明显高于电子废物回收区非职业接触者（23.2μg/g Cre）。电子废物拆解区吸烟者（23.4μg/g Cre）和不吸烟者（24.7μg/g Cre）尿液中∑10OH-PAHs 水平差异无统计学意义（p>0.05），而农村和城市参考区差异有统计学意义（p<0.05）；这表明吸烟与电子废物拆解区 PAH 暴露水平升高无关。此外，我们发现电子废物拆解区尿液中∑10OH-PAHs 和各 OH-PAHs 浓度与 8-OHdG 升高显著相关；尿液中 1-羟基芘（1-PYR）（r=0.284，p<0.01）水平与 MDA 呈显著正相关。我们的研究结果表明，电子废物拆解现场的多环芳烃暴露可能对所选参与者的 DNA 氧化损伤产生影响，但这需要在更大的研究中进行验证。
