State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Sciences and Medical Engineering, Southeast University , Nanjing 210096, China.
Collaborative Innovation Center of Suzhou Nano-Science and Technology, Suzhou Key Laboratory of Biomaterials and Technologies , Suzhou 215123, China.
ACS Appl Mater Interfaces. 2016 Jun 22;8(24):15113-9. doi: 10.1021/acsami.6b04112. Epub 2016 Jun 13.
Cell-adhesive properties are of great significance to materials serving as extracellular matrix mimics. Appropriate cell-adhesive property of material interface can balance the cell-matrix interaction and cell-cell interaction and can promote cells to form 3D structures. Herein, a novel magnetic polyacrylamide (PAM) hydrogel fabricated via combining magnetostatic field induced magnetic nanoparticles assembly and hydrogel gelation was applied as a multicellular spheroids culturing platform. When cultured on the cell-adhesive microarray interface of sliced magnetic hydrogel, normal and tumor cells from different cell lines could rapidly form multicellular spheroids spontaneously. Furthermore, cells which could only form loose cell aggregates in a classic 3D cell culture model (such as hanging drop system) were able to be promoted to form multicellular spheroids on this platform. In the light of its simplicity in fabricating as well as its effectiveness in promoting formation of multicellular spheroids which was considered as a prevailing tool in the study of the microenvironmental regulation of tumor cell physiology and therapeutic problems, this composite material holds promise in anticancer drugs or hyperthermia therapy evaluation in vitro in the future.
细胞黏附特性对于作为细胞外基质模拟物的材料非常重要。合适的材料界面的细胞黏附特性可以平衡细胞-基质相互作用和细胞-细胞相互作用，并促进细胞形成 3D 结构。在此，通过结合静磁场诱导磁性纳米粒子组装和水凝胶凝胶化制备了一种新型的磁性聚丙烯酰胺（PAM）水凝胶，用作多细胞球体培养平台。当在切片磁性水凝胶的细胞黏附微阵列界面上培养时，来自不同细胞系的正常细胞和肿瘤细胞可以迅速自发地形成多细胞球体。此外，在经典的 3D 细胞培养模型（如悬滴系统）中只能形成松散细胞聚集体的细胞，也可以在该平台上促进其形成多细胞球体。鉴于其在制备方面的简单性以及在促进多细胞球体形成方面的有效性，多细胞球体形成被认为是研究肿瘤细胞生理学和治疗问题的微环境调节的一种流行工具，这种复合材料在未来的抗癌药物或热疗评估方面具有广阔的应用前景。
