Department of Biological Sciences, University of Calgary, Calgary, AB, Canada.
Department of Chemical and Petroleum Engineering, University of Calgary, Calgary, AB, Canada.
Bioresour Technol. 2016 Sep;216:352-61. doi: 10.1016/j.biortech.2016.05.084. Epub 2016 May 26.
This study evaluated the efficacy of using a combined biofilm-biochar approach to remove organic (naphthenic acids (NAs)) and inorganic (metals) contaminants from process water (OSPW) generated by Canada's oil sands mining operations. A microbial community sourced from an OSPW sample was cultured as biofilms on several carbonaceous materials. Two biochar samples, from softwood bark (SB) and Aspen wood (N3), facilitated the most microbial growth (measured by protein assays) and were used for NA removal studies performed with and without biofilms, and in the presence and absence of contaminating metals. Similar NA removal was seen in 6-day sterile N3 and SB assays (>30%), while biodegradation by SB-associated biofilms increased NA removal to 87% in the presence of metals. Metal sorption was also observed, with up to four times more immobilization of Fe, Al, and As on biofilm-associated biochar. These results suggest this combined approach may be a promising treatment for OSPW.
本研究评估了使用组合生物膜-生物炭方法从加拿大油砂开采作业产生的工艺水（OSPW）中去除有机（环烷酸（NAs））和无机（金属）污染物的效果。从 OSPW 样本中获得的微生物群落被培养为生物膜，附着在几种碳素材料上。两种生物炭样本，一种来自软木树皮（SB），另一种来自白杨木（N3），促进了最多的微生物生长（通过蛋白质分析测量），并用于进行有和没有生物膜、有和没有污染金属存在情况下的 NA 去除研究。在无菌的 N3 和 SB 实验中（>30%），在 6 天内观察到类似的 NA 去除效果，而在存在金属的情况下，SB 相关生物膜的生物降解作用将 NA 去除率提高到 87%。也观察到金属吸附，生物膜相关生物炭对 Fe、Al 和 As 的固定率最高可达四倍。这些结果表明，这种组合方法可能是一种很有前途的 OSPW 处理方法。
