Hamey Fiona K, Nestorowa Sonia, Wilson Nicola K, Göttgens Berthold
Department of Haematology and Wellcome Trust - MRC Cambridge Stem Cell Institute, University of Cambridge, UK.
FEBS Lett. 2016 Nov;590(22):4052-4067. doi: 10.1002/1873-3468.12231. Epub 2016 Jun 21.
Haematopoietic stem and progenitor cells (HSPCs) sit at the top of the haematopoietic hierarchy, and their fate choices need to be carefully controlled to ensure balanced production of all mature blood cell types. As cell fate decisions are made at the level of the individual cells, recent technological advances in measuring gene and protein expression in increasingly large numbers of single cells have been rapidly adopted to study both normal and pathological HSPC function. In this review we emphasise the importance of combining the correct computational models with single-cell experimental techniques, and illustrate how such integrated approaches have been used to resolve heterogeneities in populations, reconstruct lineage differentiation, identify regulatory relationships and link molecular profiling to cellular function.
造血干细胞和祖细胞（HSPCs）处于造血层级的顶端，其命运选择需要得到精确控制，以确保所有成熟血细胞类型的平衡生成。由于细胞命运决定是在单个细胞层面做出的，因此，测量越来越多单个细胞中基因和蛋白质表达的最新技术进展已迅速被用于研究正常和病理性HSPC功能。在本综述中，我们强调将正确的计算模型与单细胞实验技术相结合的重要性，并举例说明这种综合方法如何用于解析群体中的异质性、重建谱系分化、识别调控关系以及将分子谱与细胞功能联系起来。
