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先进单细胞技术时代的造血。

Haematopoiesis in the era of advanced single-cell technologies.

机构信息

MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.

Haematopoietic Stem Cell Laboratory, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.

出版信息

Nat Cell Biol. 2019 Jan;21(1):2-8. doi: 10.1038/s41556-018-0227-8. Epub 2019 Jan 2.

Abstract

The molecular and functional characterization of single cells at scale has emerged as a key driver to unravel tissue biology. Thus, it is important to understand the strengths and limitations of transcriptomic approaches, molecular barcoding and functional assays used to study cellular properties at the single-cell level. Here, we review recent relevant work from the haematopoietic system and discuss how to interpret and integrate data obtained with different technologies.

摘要

大规模单细胞的分子和功能特征分析已成为揭示组织生物学的关键驱动力。因此,了解用于研究单细胞水平细胞特性的转录组学方法、分子条形码和功能测定的优缺点非常重要。在这里,我们回顾了造血系统的最新相关工作,并讨论了如何解释和整合不同技术获得的数据。

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