Institute of Environmental Science and Research Ltd., Mt Albert Science Centre, Hampstead Road, Private Bag 92-021, Auckland, New Zealand.
Forensic Sci Int Genet. 2010 Oct;4(5):311-5. doi: 10.1016/j.fsigen.2009.11.008. Epub 2010 Jan 19.
We have used the 16S-23S rRNA intergenic spacer region for identifying vaginal specific bacteria. Lactobacillus crispatus and Lactobacillus gasseri were detected in vaginal secretions but not in semen, blood or saliva. Our data indicated that both L. crispatus and L. gasseri were detected in vaginal secretions from women with different levels of expression of hormonal genes including pregnant, pre- and post-menopausal women, and a woman who has had a hysterectomy. Therefore, we have demonstrated that these Lactobacilli are promising new markers for the forensic identification of vaginal secretions. We have incorporated the Lactobacilli markers into a mRNA multiplex system to produce an 11-plex assay that can identify circulatory blood, menstrual blood, saliva, semen (in the presence and absence of spermatozoa) and vaginal secretions.
我们使用 16S-23S rRNA 基因间隔区来鉴定阴道特定细菌。阴道分泌物中可检测到乳酸杆菌 crispatus 和乳酸杆菌 gasseri，但精液、血液或唾液中则无法检测到。我们的数据表明，无论是在激素基因表达水平不同的女性（包括孕妇、绝经前和绝经后女性，以及已接受子宫切除术的女性）的阴道分泌物中，均能检测到 L. crispatus 和 L. gasseri。因此，我们证明这些乳酸菌是法医鉴定阴道分泌物的有前途的新型标志物。我们将乳酸菌标志物纳入 mRNA 多重检测系统中，开发出 11 重检测方法，可以识别循环血液、月经血、唾液、精液（有或无精子）和阴道分泌物。
