小檗果提取物对大鼠百草枯诱导的肺纤维化的保护作用。
Protective effect of Berberis vulgaris fruit extract against Paraquat-induced pulmonary fibrosis in rats.
机构信息
Department of Internal Medicine, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Biomed Pharmacother. 2016 Jul;81:329-336. doi: 10.1016/j.biopha.2016.04.027. Epub 2016 Apr 26.
BACKGROUND
Pulmonary fibrosis induced by paraquat (PQ) has caused a large number of human fatalities all over the world, especially in Asian region. The main potential mechanism of PQ toxicity has been thought to be mediated by ROS. The present study was designed to evaluate the efficacy of the Berberis vulgaris fruit extract (BVFE) against PQ-induced pulmonary fibrosis in rats.
METHODS
Forty male rats were randomly divided into five experimental groups each containing eight rats. Groups 1 and 2, served as a negative and positive control and received a single dose of intratracheal instillation of saline and PQ (20mg/kg), respectively. Groups 3-5 were treated with different doses of BVFE (100, 200, 400mg/kg/day, orally) 1 week before the PQ injection and continued for 3 weeks. The rats were sacrificed 21days after PQ. Malondialdehyde (MDA), Hydroxyproline, inflammatory and fibrogenic cytokine tumor necrosis factor (TNF)-α, interleukin (IL)-6 and transforming growth factor (TGF)-β1 in lung tissue were determined. Presence of fibrosis, inflammatory cells, connective tissue and collagen deposition in lung were evaluated microscopically by hematoxylin and eosin (H&E) staining. Dried extract was standardized by amount of berberine by HPTLC methods by silica gel plate.
RESULTS
The results showed that PQ could significantly increase the lung MDA, hydroxyproline, TNF-α, IL-6 and TGF-β1 levels. BVFE ameliorated the biochemical and histological lung alterations induced by PQ.
CONCLUSIONS
The present study indicates the hydroalcolic extract of Berberis vulgaris fruit has beneficial effects in rat pulmonary fibrosis induced by PQ in a dose-dependent manner, possibly by anti-oxidant and anti- inflammatory properties, which might be due to its berberine alkaloid content.
背景
百草枯（PQ）引起的肺纤维化已在全球范围内导致大量人类死亡，尤其是在亚洲地区。PQ 毒性的主要潜在机制被认为是由 ROS 介导的。本研究旨在评估小檗果提取物（BVFE）对大鼠 PQ 诱导的肺纤维化的疗效。
方法
40 只雄性大鼠随机分为 5 个实验组，每组 8 只。第 1 组和第 2 组分别作为阴性和阳性对照组，接受单次气管内滴注生理盐水和 PQ（20mg/kg）。第 3-5 组在 PQ 注射前 1 周开始用不同剂量的 BVFE（100、200、400mg/kg/天，口服）治疗，并持续 3 周。大鼠在 PQ 注射后 21 天处死。测定肺组织丙二醛（MDA）、羟脯氨酸、炎症和纤维化细胞因子肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6 和转化生长因子（TGF）-β1。用苏木精和伊红（H&E）染色法通过肺组织的纤维化、炎症细胞、结缔组织和胶原蛋白沉积来评估显微镜下的存在。通过硅胶板的 HPTLC 方法，用含量的小檗碱对干提取物进行标准化。
结果
结果表明，PQ 可显著增加肺 MDA、羟脯氨酸、TNF-α、IL-6 和 TGF-β1 水平。BVFE 改善了 PQ 引起的生化和组织学肺改变。
结论
本研究表明，小檗果的水醇提取物以剂量依赖的方式对 PQ 诱导的大鼠肺纤维化具有有益作用，可能通过抗氧化和抗炎特性，这可能与其小檗碱生物碱含量有关。
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