Danaei Nazanin, Sadeghi Heibatollah, Asfarm Arash, Rostamzadeh Davoud, Panahi Kokhdan Esmaeel, Sadeghi Hossein, Rahimi Negin
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Tehran University of Medical Sciences, Tehran, Iran.
Heliyon. 2023 Aug 20;9(8):e19236. doi: 10.1016/j.heliyon.2023.e19236. eCollection 2023 Aug.
Pulmonary fibrosis (PF) is a chronic and progressive respiratory disease representing the final stage of lung inflammatory disorders. Reactive oxygen species (ROS), an essential factor in the formation and progression of pulmonary fibrosis, are a significant adverse effect of Bleomycin (BLM). Antioxidant activities have been found in . In this study, we attempted to explore the function of hydroalcoholic extract of () and Betulin in inhibiting bleomycin (BLM)-induced pulmonary fibrosis in rat".
The current experimental study used 36 male Wistar rats (180-220). Following a random process, the animals were divided into six groups six (n = 6). Group, I (the control group) received normal saline, while Group II (the hazardous group) received intratracheal BLM (7.5 units per kg). Following the administration of BLM, Groups V and VI received daily doses of vitamin E (500 mg/kg/d, p.o.) and Betulin (10 mg kg/d, p.o.), whereas Groups III and IV received daily doses of extract (300 and 600 mg/kg/d, p.o.). Then, blood samples from the hearts of the animals were taken to assess the plasma concentrations of nitric oxide (NO) and malondialdehyde (MDA). Finally, the rats were euthanized, and the lung tissues were taken out for histological analysis and assessments of the levels of lung hydroxyproline (HP), ferric-reducing ability (FRAP), NO, Glutathione Concentration (GSH), thiol content (tSH) and MDA.
Elevated lung index, lung hydroxyproline, NO, and MDA plasma levels, and a reduction in total body thiol content (tSH) in the group receiving BLM were evidence of pulmonary toxicity. Treatment with extracts, Betulin, and Vit E, especially at 600 mg/kg, led to a marked reduction in the above parameters compared with the BLM-received group (p < 0.01). Histological Analysis of the BLM-treated group showed a considerable Lung injury with interstitial infiltration, collapsed alveolar spaces, and alveolar septal thickening. These changes were mitigated with 600, Betulin-, and vitamin E. These changes were mitigated with 600, Betulin-, and vitamin E.
These findings suggest that and Betulin prevent bleomycin-induced lung fibrosis in rats by decreasing inflammatory and antioxidant markers. , therefore, have the potential to be used therapeutically to treat pulmonary fibrosis.
肺纤维化（PF）是一种慢性进行性呼吸系统疾病，代表着肺部炎症性疾病的终末期。活性氧（ROS）是肺纤维化形成和进展的关键因素，是博来霉素（BLM）的一种显著不良反应。已在……中发现抗氧化活性。在本研究中，我们试图探究[具体植物名称]水醇提取物和桦木醇在抑制博来霉素（BLM）诱导的大鼠肺纤维化中的作用。
当前的实验研究使用了36只雄性Wistar大鼠（体重180 - 220克）。经过随机分组，将动物分为六组（每组n = 6）。第一组（对照组）接受生理盐水，而第二组（损伤组）接受气管内注射BLM（每千克7.5单位）。在给予BLM后，第五组和第六组每天分别给予维生素E（500毫克/千克/天，口服）和桦木醇（10毫克/千克/天，口服），而第三组和第四组每天分别给予[具体植物名称]提取物（300和600毫克/千克/天，口服）。然后，采集动物心脏的血液样本以评估血浆中一氧化氮（NO）和丙二醛（MDA）的浓度。最后，对大鼠实施安乐死，并取出肺组织进行组织学分析以及评估肺羟脯氨酸（HP）、铁还原能力（FRAP）、NO、谷胱甘肽浓度（GSH）、硫醇含量（tSH）和MDA的水平。
接受BLM的组中肺指数、肺羟脯氨酸、NO和血浆MDA水平升高，以及全身硫醇含量（tSH）降低，这些都是肺毒性的证据。与接受BLM的组相比，用[具体植物名称]提取物、桦木醇和维生素E治疗，尤其是600毫克/千克剂量时，上述参数显著降低（p < 0.01）。对接受BLM治疗组的组织学分析显示有明显的肺损伤，伴有间质浸润、肺泡腔塌陷和肺泡间隔增厚。用600毫克/千克剂量的[具体植物名称]提取物、桦木醇和维生素E治疗可减轻这些变化。
这些发现表明，[具体植物名称]提取物和桦木醇通过降低炎症和抗氧化标志物来预防博来霉素诱导的大鼠肺纤维化。因此，[具体植物名称]提取物有潜力用于治疗肺纤维化。
