生物合成基因簇的拷贝数对异源宿主中抗生素产量水平的影响。
An influence of the copy number of biosynthetic gene clusters on the production level of antibiotics in a heterologous host.
作者信息
Manderscheid Niko, Bilyk Bohdan, Busche Tobias, Kalinowski Jörn, Paululat Thomas, Bechthold Andreas, Petzke Lutz, Luzhetskyy Andriy
机构信息
Helmholtz-Institute for Pharmaceutical Research Saarland, Saarland University, Campus C2.3, Saarbrücken 66123, Germany.
PharmBioTec GmbH, Science Park 1, 66123 Saarbrücken, Germany.
出版信息
J Biotechnol. 2016 Aug 20;232:110-7. doi: 10.1016/j.jbiotec.2016.05.038. Epub 2016 Jun 2.
Streptomyces albus J1074 is a well-known host for heterologous expression of secondary metabolites. To further increase its potential and to study the influence of cluster multiplication, additional φC31-attachment site was integrated into its genome using a system for transposon mutagenesis. Four secondary metabolite clusters were expressed in strains with different numbers of attachment sites, ranging from one to three copies of the site. Secondary metabolite production was examined and a new compound could be detected, purified and its structure was elucidated.
白色链霉菌J1074是一种用于次生代谢产物异源表达的著名宿主。为了进一步提高其潜力并研究基因簇倍增的影响，利用转座子诱变系统将额外的φC31附着位点整合到其基因组中。在具有不同数量附着位点（从一个到三个拷贝）的菌株中表达了四个次生代谢产物基因簇。检测了次生代谢产物的产生情况，检测到一种新化合物并进行了纯化，阐明了其结构。
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