Laballe Renaud, Vigne Jonathan, Denion Eric, Lemaitre Florian, Goux Didier, Pisella Pierre-Jean
Caen University Hospital, Department of Ophthalmology, Caen, F-14033, France.
Caen University Hospital, Department of Pharmacy, Caen, F-14033, France.
Cont Lens Anterior Eye. 2016 Oct;39(5):394-6. doi: 10.1016/j.clae.2016.04.008. Epub 2016 Jun 2.
Bacterial keratitis is a sight threatening infection of the cornea which remains one of the most important potential complications of contact lens use. If the corneal ulcer is small, peripheral with no impending perforation present, intensive monotherapy with fluoroquinolones could be used. Therefore, a study was conducted with the objective to provide pharmacological data of the intra-ocular diffusion after administration of Ofloxacin using a scleral lens reservoir, as well as an evaluation of surface tolerability in rabbits.
Samples of corneas, aqueous humor and vitreous were collected to measure the drug levels of Ofloxacin using High Performance Liquid Chromatography. The corneas were examined by electron microscopy scanning and the eyeballs by light polarizing microscopy in order to evaluate surface tolerability.
Ofloxacin levels found in the aqueous humor and cornea were higher than those previously reported. The mean Ofloxacin corneal levels exceeded the MIC (Minimum Inhibitory Concentration) for which 90% of isolates are indicated for all bacteria implicated in keratitis.
To our knowledge, this is the first preclinical study assessing local tolerance and intra-ocular diffusion of Ofloxacin after administration using a scleral lens reservoir.
细菌性角膜炎是一种威胁视力的角膜感染，仍然是隐形眼镜使用最重要的潜在并发症之一。如果角膜溃疡较小、位于周边且无即将穿孔的情况，可使用氟喹诺酮类药物进行强化单一疗法。因此，进行了一项研究，目的是提供使用巩膜镜储液器给药后氧氟沙星眼内扩散的药理学数据，以及对兔眼表面耐受性的评估。
采集角膜、房水和玻璃体样本，使用高效液相色谱法测量氧氟沙星的药物水平。通过电子显微镜扫描检查角膜，通过光偏振显微镜检查眼球，以评估表面耐受性。
房水和角膜中发现的氧氟沙星水平高于先前报道的水平。氧氟沙星角膜平均水平超过了对所有与角膜炎相关细菌中90%分离株有抑制作用的最低抑菌浓度（MIC）。
据我们所知，这是第一项评估使用巩膜镜储液器给药后氧氟沙星局部耐受性和眼内扩散的临床前研究。
