Regazzoni Luca, de Courten Barbora, Garzon Davide, Altomare Alessandra, Marinello Cristina, Jakubova Michaela, Vallova Silvia, Krumpolec Patrik, Carini Marina, Ukropec Jozef, Ukropcova Barbara, Aldini Giancarlo
Department of Pharmaceutical Sciences, Università degli Studi di Milano, Milan, Italy.
Monash Centre for Health, Research and Implementation, School of Public health and Preventive Medicine, Melbourne, Australia.
Sci Rep. 2016 Jun 6;6:27224. doi: 10.1038/srep27224.
Carnosine is a natural dipeptide able to react with reactive carbonyl species, which have been recently associated with the onset and progression of several human diseases. Herein, we report an intervention study in overweight individuals. Carnosine (2 g/day) was orally administered for twelve weeks in order to evaluate its bioavailability and metabolic fate. Two carnosine adducts were detected in the urine samples of all subjects. Such adducts are generated from a reaction with acrolein, which is one of the most toxic and reactive compounds among reactive carbonyl species. However, neither carnosine nor adducts have been detected in plasma. Urinary excretion of adducts and carnosine showed a positive correlation although a high variability of individual response to carnosine supplementation was observed. Interestingly, treated subjects showed a significant decrease in the percentage of excreted adducts in reduced form, accompanied by a significant increase of the urinary excretion of both carnosine and carnosine-acrolein adducts. Altogether, data suggest that acrolein is entrapped in vivo by carnosine although the response to its supplementation is possibly influenced by individual diversities in terms of carnosine dietary intake, metabolism and basal production of reactive carbonyl species.
肌肽是一种天然二肽，能够与活性羰基物质发生反应，而活性羰基物质最近被认为与多种人类疾病的发生和发展有关。在此，我们报告了一项针对超重个体的干预研究。口服给予肌肽（2克/天），持续12周，以评估其生物利用度和代谢命运。在所有受试者的尿液样本中检测到两种肌肽加合物。这些加合物是由与丙烯醛反应生成的，丙烯醛是活性羰基物质中毒性和反应性最强的化合物之一。然而，在血浆中未检测到肌肽和加合物。加合物和肌肽的尿排泄呈正相关，尽管观察到个体对肌肽补充的反应存在很大差异。有趣的是，接受治疗的受试者排泄的还原形式加合物百分比显著降低，同时肌肽和肌肽 - 丙烯醛加合物的尿排泄量显著增加。总之，数据表明肌肽在体内捕获了丙烯醛，尽管其补充反应可能受肌肽饮食摄入量、代谢以及活性羰基物质基础生成方面的个体差异影响。
