Oncel Mufide, Akbulut Seval, Toka Ozer Turkan, Kiyici Aysel, Keles Mustafa, Baltaci Beyhan, Turk Suleyman
a Department of Biochemistry , Mevlana University Medical Faculty , Konya , Turkey ;
b Department of Biochemistry , Nevsehir State Hospital , Nevsehir , Turkey ;
Ren Fail. 2016 Aug;38(7):1071-5. doi: 10.1080/0886022X.2016.1193874. Epub 2016 Jun 7.
Cytokines are essential mediators of immune response. Chronic renal failure patients suffer from chronic inflammation that results from factors such as impaired renal function, accumulation of uremic toxins and bio incompatibility of dialyzer membranes. These patients are also at increased risk of cardiovascular diseases. We have evaluated cytokines, adipocytokines and inflammatory markers in patients with chronic renal failure undergoing hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD).
We have determined serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), high-sensitivity C-reactive protein (hsCRP), leptin and ghrelin levels of chronic renal failure patients treated with either HD (n = 20) or CAPD (n = 20). TNF-α, IL-6, ghrelin and leptin measurements were performed by commercially available kits based on enzyme-linked immunosorbent assay (ELISA) method. hsCRP levels were determined by turbidimetric methods.
Serum TNF-α and IL-6 levels of patients on HD were significantly higher than those of the ones on CAPD (p < 0.05). Ghrelin, leptin and hsCRP concentrations were similar in both groups.
We can conclude that cytokine production is more obvious in HD process.
细胞因子是免疫反应的重要介质。慢性肾衰竭患者患有慢性炎症，其由诸如肾功能受损、尿毒症毒素积累和透析器膜的生物不相容性等因素引起。这些患者患心血管疾病的风险也增加。我们评估了接受血液透析（HD）和持续性非卧床腹膜透析（CAPD）的慢性肾衰竭患者的细胞因子、脂肪细胞因子和炎症标志物。
我们测定了接受HD（n = 20）或CAPD（n = 20）治疗的慢性肾衰竭患者的血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、高敏C反应蛋白（hsCRP）、瘦素和胃饥饿素水平。TNF-α、IL-6、胃饥饿素和瘦素的测量通过基于酶联免疫吸附测定（ELISA）方法的商用试剂盒进行。hsCRP水平通过比浊法测定。
HD患者的血清TNF-α和IL-6水平显著高于CAPD患者（p < 0.05）。两组的胃饥饿素、瘦素和hsCRP浓度相似。
我们可以得出结论，HD过程中细胞因子的产生更为明显。
