Department of Physics, Indian Institute of Technology Delhi, New Delhi 110016, India.
Department of Physics, Indian Institute of Technology Delhi, New Delhi 110016, India.
Biosens Bioelectron. 2016 Nov 15;85:986-995. doi: 10.1016/j.bios.2016.05.082. Epub 2016 May 28.
In this piece of work, a fiber optic sensor has been fabricated and characterized using surface plasmon resonance for dextrose sensing. The concentration range used in this study is for diagnosing the cases of hypoglycaemia especially in suppression tests of insulinoma. Insulinoma is a medical case in which the person is recognized being hypoglycaemic with the blood dextrose level falling down to 2.2mM or less. Thus, the sensor has been characterized for the dextrose concentration range of 0 mM-10mM including the cases of normal blood dextrose range. Coatings of silver layer and zinc oxide nanorods have been carried out on the bare core fiber with a dual role of zinc oxide followed by immobilization of glucose oxidase. A three stage optimization procedure has been adopted for the best performance of the sensor. Absorbance spectra have been plotted and peak absorbance wavelengths have been extracted for each concentration chosen along with the sensitivities. The results have been made conclusive with control experiments. The probe has also been tested on sample having blood serum to check the reliability of the sensor. The sensor shows better selectivity and response time along with its real time applications, online monitoring, remote sensing and reusability.
在这项工作中，我们使用表面等离子体共振制造并表征了一种光纤传感器，用于葡萄糖感测。本研究使用的浓度范围是为了诊断低血糖症病例，特别是在胰岛素瘤抑制试验中。胰岛素瘤是一种病症，其特征是患者血糖水平下降到 2.2mM 或更低，被认为患有低血糖症。因此，该传感器已针对 0mM-10mM 的葡萄糖浓度范围进行了表征，包括正常血糖范围的情况。银层和氧化锌纳米棒的涂层已经在裸芯光纤上进行，氧化锌具有双重作用，随后固定葡萄糖氧化酶。采用了三阶段优化程序，以获得传感器的最佳性能。绘制了吸收光谱，并提取了所选每个浓度的峰值吸收波长及其灵敏度。结果与对照实验进行了对比。该探头还在含有血清的样本上进行了测试，以检查传感器的可靠性。该传感器具有更好的选择性和响应时间，以及其实时应用、在线监测、远程传感和可重复使用性。
