从希氏乳杆菌中分离出的革兰氏阳性广宿主范围质粒的特性分析。
Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii.
作者信息
Josson K, Scheirlinck T, Michiels F, Platteeuw C, Stanssens P, Joos H, Dhaese P, Zabeau M, Mahillon J
机构信息
Plant Genetic Systems, Ghent, Belgium.
出版信息
Plasmid. 1989 Jan;21(1):9-20. doi: 10.1016/0147-619x(89)90082-6.
Two plasmids, pLAB1000 and pLAB2000 (3.3 and 9.1 kb, respectively), have been isolated from a grass silage strain of Lactobacillus hilgardii. Both plasmids were cloned in Escherichia coli and characterized through restriction mapping. A 1.6-kb XbaI-SacI fragment of pLAB1000 appeared to be sufficient for autonomous replication in Lactobacillus plantarum and in Bacillus subtilis. Different shuttle vectors for E. coli and gram-positive bacteria were developed using the pLAB1000 plasmid. These could stably be maintained in Lactobacillus, Enterococcus, and Bacillus under selective conditions. Plasmids sharing DNA homologies with pLAB1000 have been observed in different strains of the related species L. plantarum.
从希氏乳杆菌的青贮饲料菌株中分离出了两个质粒，分别为pLAB1000和pLAB2000（大小分别为3.3 kb和9.1 kb）。这两个质粒都在大肠杆菌中进行了克隆，并通过限制性酶切图谱进行了表征。pLAB1000的一个1.6 kb XbaI-SacI片段似乎足以在植物乳杆菌和枯草芽孢杆菌中自主复制。利用pLAB1000质粒开发了不同的大肠杆菌和革兰氏阳性菌穿梭载体。在选择性条件下，这些载体能够在乳杆菌属、肠球菌属和芽孢杆菌属中稳定维持。在相关物种植物乳杆菌的不同菌株中也观察到了与pLAB1000具有DNA同源性的质粒。
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